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Journal Club
Page no: 3
Journal Club, page 3
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Antibiotic Resistant Strains Within the Human Microbiome
Antibiotic Resistance
Carbapenemase-Resistant Enterobacterales (CRE)
20 Nov 2025
Lora Benoit Ph.D
Colonization of the human gut by antibiotic-resistant strains is strain‐ and context‐specific rather than singularly driven by antibiotic selection.
Microbiome
Rapid Point-of-Care Detection of STIs CT/NG/MG
Chlamydia
Mycoplasma genitalium
Neisseria
20 Nov 2025
Lora Benoit Ph.D
The Cobas Liat molecular POC assay delivers rapid results in 20 min and specificities >97% for
Chlamydia trachomatis
,
Neisseria gonorrhoeae,
and
Mycoplasma genitalium
(MG), and sensitivities ≥92% regardless of symptomatic status.
Clinical & Veterinary
Syndromic Respiratory Pathogen Panel Multicenter Evaluation
Bordatella
Diagnostic Systems
Influenza
20 Nov 2025
Lora Benoit Ph.D
A multicenter evaluation confirms that the LIAISON PLEX Respiratory Flex Assay delivers robust, clinically reliable detection of up to 19 respiratory pathogens, supporting its integration into routine diagnostic use.
Clinical & Veterinary
Listeria monocytogenes
Biofilm Resistance to Standard Sanitizers
Biofilm
Journal Club
Listeria monocytogenes
19 Nov 2025
Lora Benoit Ph.D
Lucy Sutton
et al.
(2025) show that mature biofilms of L. monocytogenes on stainless‐steel surfaces failed to respond to high‐concentration sanitizers.
Food & Beverage
Spray-on Biocide Durability Claims a Bust in the Real World
Sanitizers
Surface Monitoring
18 Nov 2025
Lora Benoit Ph.D
Commercially available spray-on surface biocide (SAQAS) showed no significant antimicrobial effect at either 5- or 30-day post-application under real world conditions.
Food & Beverage
Clinical & Veterinary
Using Microbial Ecology to Strengthen HACCP Sanitation Controls
HACCP
Journal Club
Sanitizers
18 Nov 2025
Lora Benoit Ph.D
Yadav
et al.
(2025) assessed how the multi-stage sanitation process impacts microbial populations in a large commercial beef-processing facility by tracking total aerobic counts,
Enterobacteriacea
e/coliforms, and microbial composition via sequencing.
Food & Beverage
Microbiome-informed Diagnostic for
Clostridioides difficile
Infection
Clostridioides difficle
17 Nov 2025
Lora Benoit Ph.D
Gut microbiome profiles can be used to distinguish
C. difficile
colonized patients with diarrhea from those with true CDI infections.
Clinical & Veterinary
Enhanced Testing and Surveillance of Cryptosporidium
Cryptosporidium/Giardia
Journal Club
17 Nov 2025
Lora Benoit Ph.D
An optimized molecular-based workflow for detecting
Cryptosporidium
oocysts across complex agricultural matrices is described. The system integrates ddPCR with improved sample preparation and DNA recovery.
Food & Beverage
Water
Multiplex PCR Detects Resistance Genes from Blood Cultures
Antibiotic Resistance
Blood Culture
Journal Club
17 Nov 2025
Lora Benoit Ph.D
Marxreiter
et al.
(2025) benchmarked a rapid multiplex PCR assay against microdilution susceptibility testing, demonstrating how effectively molecular detection of resistance genes mirrors phenotypic resistance in Gram-negative pathogens.
Clinical & Veterinary
Comparison of Three Rapid Antimicrobial-Susceptibility-Testing Systems
Antibiotic Resistance
Antimicrobial Susceptibility Testing
Journal Club
17 Nov 2025
Lora Benoit Ph.D
Squitieri
et al
. (2025) have conducted a head-to-head comparison of 3 rapid antimicrobial-susceptibility-testing (AST) systems applied directly to Gram-negative blood cultures in a clinical microbiology setting.
Clinical & Veterinary
Rapid Serotyping of
Salmonella
via MALDI-TOF and Machine Learning
Artifical Intelligence
Journal Club
MALDI-TOF
05 Nov 2025
Lora Benoit Ph.D
Study shows that combining MALDI-TOF MS with machine learning (XGBoost) can accurately identify
Salmonella
serotypes. This combined approach might soon provide diagnostic labs with a rapid and cost-effective approach to resolving
Salmonella
serotypes.
Food & Beverage
Clinical & Veterinary
What Microbiology Labs Need to Know About Updates to Antimicrobial Resistance Testing in CLSI M100
Antibiotic Resistance
Journal Club
15 Oct 2025
Lora Benoit Ph.D
In the new CLSI M100 edition, major updates were introduced - including restructured tables, new breakpoints, and a tiered approach to reporting.
Clinical & Veterinary
Everything You Need to Know About Equipment Validation for Sterility Testing
ATMP - Advanced Therapeutic Medicinal Products
Cell and Gene Therapies
Journal Club
14 Oct 2025
Lora Benoit Ph.D
As novel biologics and cell therapies advance, sterility testing is becoming increasingly complex and subject to cGMP scrutiny. Gebo
et al.
outline how clinical microbiology labs - traditionally CLIA/CAP-regulated - can align with FDA, USP, and ISO expectations.
Clinical & Veterinary
Pharmaceutical
How
Klebsiella pneumoniae
Evolves and Persists in the Clinical Setting
Journal Club
Klebsiella pneumoniae carbapenemase (KPC)
13 Oct 2025
Lora Benoit Ph.D
Study on
Klebsiella pneumoniae
clone that evolved within patients over a 5-year hospital outbreak: virulence, capsule, iron scavenging, and biofilms shifted independently across hosts - highlighting how multidrug-resistant pathogens rapidly adapt and persist under clinical pressures.
Clinical & Veterinary
Tracing 20 Years of
Salmonella
Dublin Evolution: Human, Animal, Environmental Sources
Journal Club
Salmonella
04 Nov 2025
Lora Benoit Ph.D
In one of the most comprehensive studies of its kind, a genomic analysis of 2,150
Salmonella
Dublin isolates collected in the U.S. over 20 years reveals strong genetic similarity among isolates from human, cattle, and environmental reservoirs.
Clinical & Veterinary
Legionella
Detection in Water: Evaluating ISO 11731:2017 - Are All Methods the Same?
ISO 11731 - Legionella
Legionella spp.
04 Nov 2025
Lora Benoit Ph.D
A multicenter study systematically compared six ISO 11731:2017 standardized culture techniques for detecting
Legionella
in 276 hospital hot-water samples. Detection rates varied significantly depending on the method used.
Water
A Defined Microbial Consortium Replicates Fine-flavor Chocolate Fermentation Traits
Journal Club
03 Nov 2025
Lora Benoit Ph.D
A rationally defined, 7-member microbial consortium assembled from isolated yeast and bacterial strains found during spontaneous cocoa fermentation was found to faithfully reproduce the chemical, sensory, and microbiological attributes of traditional fine-flavor chocolate.
Food & Beverage
Design of a Host-directed Adjuvant to Sensitize Intracellular Bacterial Persisters to Antibiotics
Antibiotic Resistance
Mycobacterium tuberculosis
Salmonella Typhimurium
03 Nov 2025
Lora Benoit Ph.D
Lu
et al.
, (2025) describes the discovery and characterization of a non-toxic small-molecule adjuvant, K1, which modulates the host cell in such a way as to resuscitate metabolically dormant intracellular bacteria making them become sensitive to antibiotics.
Clinical & Veterinary
Comparison of cgMLST Pipelines for Hospital Outbreak Detections
Whole Genome Sequencing
03 Nov 2025
Lora Benoit Ph.D
Three commercial cgMLST pipelines were compared, using 255 hospital outbreak isolates, evaluating their concordance in identifying outbreak-related clusters and whether they consistently distinguish “clustered” vs “non-clustered” isolate pairs.
Clinical & Veterinary
Phage Cocktail Tailored to Treat Hospital Acquired
Enterobacter cloacae
Complex Infections
Coliphages
Enterobacter Cloacae Complex (ECC)
ESKAPE Pathogens
03 Nov 2025
Lora Benoit Ph.D
Study demonstrates that an institution-specific phage cocktail, built using local strain surveillance, can reduce time-to-effective therapy, curb transmission of resistant clones, and limit off-target ecological effects compared with broad, non-targeted antimicrobial agents.
Clinical & Veterinary
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