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Antibiotic Resistant Strains Within the Human Microbiome

Anaerobic Human Gut microbiome Microcosms
Antibiotic ResistanceCarbapenemase-Resistant Enterobacterales (CRE)
20 Nov 2025 Lora Benoit Ph.D
Colonization of the human gut by antibiotic-resistant strains is strain‐ and context‐specific rather than singularly driven by antibiotic selection.
Microbiome

Rapid Point-of-Care Detection of STIs CT/NG/MG

Study Supports Shift Toward POC Molecular Diagnostics for STIs
ChlamydiaMycoplasma genitaliumNeisseria
20 Nov 2025 Lora Benoit Ph.D
The Cobas Liat molecular POC assay delivers rapid results in 20 min and specificities >97% for Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycoplasma genitalium (MG), and sensitivities ≥92% regardless of symptomatic status.
Clinical & Veterinary

Syndromic Respiratory Pathogen Panel Multicenter Evaluation

Study on LIAISON PLEX Respiratory Flex Assay
BordatellaDiagnostic SystemsInfluenza
20 Nov 2025 Lora Benoit Ph.D
A multicenter evaluation confirms that the LIAISON PLEX Respiratory Flex Assay delivers robust, clinically reliable detection of up to 19 respiratory pathogens, supporting its integration into routine diagnostic use.
Clinical & Veterinary

Listeria monocytogenes Biofilm Resistance to Standard Sanitizers

Listeria monocytogenes in Biofilms
BiofilmJournal ClubListeria monocytogenes
19 Nov 2025 Lora Benoit Ph.D
Lucy Sutton et al. (2025) show that mature biofilms of L. monocytogenes on stainless‐steel surfaces failed to respond to high‐concentration sanitizers. 
Food & Beverage

Spray-on Biocide Durability Claims a Bust in the Real World

Study on Effects of Spray-on Biocide
SanitizersSurface Monitoring
18 Nov 2025 Lora Benoit Ph.D
Commercially available spray-on surface biocide (SAQAS) showed no significant antimicrobial effect at either 5- or 30-day post-application under real world conditions. 
Food & Beverage Clinical & Veterinary

Using Microbial Ecology to Strengthen HACCP Sanitation Controls

Factors Influencing Sanitizer Effectiveness
HACCPJournal ClubSanitizers
18 Nov 2025 Lora Benoit Ph.D
Yadav et al. (2025) assessed how the multi-stage sanitation process impacts microbial populations in a large commercial beef-processing facility by tracking total aerobic counts, Enterobacteriaceae/coliforms, and microbial composition via sequencing.
Food & Beverage

Microbiome-informed Diagnostic for Clostridioides difficile Infection

Clostridioides difficile
Clostridioides difficle
17 Nov 2025 Lora Benoit Ph.D
Gut microbiome profiles can be used to distinguish C. difficile colonized patients with diarrhea from those with true CDI infections. 
Clinical & Veterinary

Enhanced Testing and Surveillance of Cryptosporidium

Cryptosporidium oocytes
Cryptosporidium/GiardiaJournal Club
17 Nov 2025 Lora Benoit Ph.D
An optimized molecular-based workflow for detecting Cryptosporidium oocysts across complex agricultural matrices is described. The system integrates ddPCR with improved sample preparation and DNA recovery.
Food & Beverage Water

Multiplex PCR Detects Resistance Genes from Blood Cultures

Detection of Resistance Genes in Blood Cultures by Multiplex PCR
Antibiotic ResistanceBlood CultureJournal Club
17 Nov 2025 Lora Benoit Ph.D
Marxreiter et al. (2025) benchmarked a rapid multiplex PCR assay against microdilution susceptibility testing, demonstrating how effectively molecular detection of resistance genes mirrors phenotypic resistance in Gram-negative pathogens.
Clinical & Veterinary

Comparison of Three Rapid Antimicrobial-Susceptibility-Testing Systems

Comparison of Rapid AST Systems like VITEK REVEAL
Antibiotic ResistanceAntimicrobial Susceptibility TestingJournal Club
17 Nov 2025 Lora Benoit Ph.D
Squitieri et al. (2025) have conducted a head-to-head comparison of 3 rapid antimicrobial-susceptibility-testing (AST) systems applied directly to Gram-negative blood cultures in a clinical microbiology setting.
Clinical & Veterinary

Rapid Serotyping of Salmonella via MALDI-TOF and Machine Learning

Serotyping Salmonella Using MALDI-TOF and AI
Artifical IntelligenceJournal ClubMALDI-TOF
05 Nov 2025 Lora Benoit Ph.D
Study shows that combining MALDI-TOF MS with machine learning (XGBoost) can accurately identify Salmonella serotypes. This combined approach might soon provide diagnostic labs with a rapid and cost-effective approach to resolving Salmonella serotypes.
Food & Beverage Clinical & Veterinary

What Microbiology Labs Need to Know About Updates to Antimicrobial Resistance Testing in CLSI M100

Antimicrobial Resistance Testing in CLSI M100
Antibiotic ResistanceJournal Club
15 Oct 2025 Lora Benoit Ph.D
In the new CLSI M100 edition, major updates were introduced - including restructured tables, new breakpoints, and a tiered approach to reporting. 
Clinical & Veterinary

Everything You Need to Know About Equipment Validation for Sterility Testing

Sterility Testing of Cell and Gene Therapy Products
ATMP - Advanced Therapeutic Medicinal ProductsCell and Gene TherapiesJournal Club
14 Oct 2025 Lora Benoit Ph.D
As novel biologics and cell therapies advance, sterility testing is becoming increasingly complex and subject to cGMP scrutiny. Gebo et al. outline how clinical microbiology labs - traditionally CLIA/CAP-regulated - can align with FDA, USP, and ISO expectations.
Clinical & Veterinary Pharmaceutical

How Klebsiella pneumoniae Evolves and Persists in the Clinical Setting

How K pneumoniae Evolves and Persists in Clinical Setting
Journal ClubKlebsiella pneumoniae carbapenemase (KPC)
13 Oct 2025 Lora Benoit Ph.D
Study on Klebsiella pneumoniae clone that evolved within patients over a 5-year hospital outbreak: virulence, capsule, iron scavenging, and biofilms shifted independently across hosts - highlighting how multidrug-resistant pathogens rapidly adapt and persist under clinical pressures.
Clinical & Veterinary

Tracing 20 Years of Salmonella Dublin Evolution: Human, Animal, Environmental Sources

Salmonella Spread Risk From Livestock
Journal ClubSalmonella
04 Nov 2025 Lora Benoit Ph.D
In one of the most comprehensive studies of its kind, a genomic analysis of 2,150 Salmonella Dublin isolates collected in the U.S. over 20 years reveals strong genetic similarity among  isolates from human, cattle, and environmental reservoirs. 
Clinical & Veterinary

Legionella Detection in Water: Evaluating ISO 11731:2017 - Are All Methods the Same?

Best Way to Detect Legionella in Water
ISO 11731 - LegionellaLegionella spp.
04 Nov 2025 Lora Benoit Ph.D
A multicenter study systematically compared six ISO 11731:2017 standardized culture techniques for detecting Legionella in 276 hospital hot-water samples.  Detection rates varied significantly depending on the method used.
Water

A Defined Microbial Consortium Replicates Fine-flavor Chocolate Fermentation Traits

Precision Fermentation Produces Fine Chocolate Flavour
Journal Club
03 Nov 2025 Lora Benoit Ph.D
A rationally defined, 7-member microbial consortium assembled from isolated yeast and bacterial strains found during spontaneous cocoa fermentation was found to faithfully reproduce the chemical, sensory, and microbiological attributes of traditional fine-flavor chocolate. 
Food & Beverage

Design of a Host-directed Adjuvant to Sensitize Intracellular Bacterial Persisters to Antibiotics

Intracellular Bacterial Persister Cells Contribute to Antibiotic Failure
Antibiotic ResistanceMycobacterium tuberculosisSalmonella Typhimurium
03 Nov 2025 Lora Benoit Ph.D
Lu et al., (2025) describes the discovery and characterization of a non-toxic small-molecule adjuvant, K1, which modulates the host cell in such a way as to resuscitate metabolically dormant intracellular bacteria making them become sensitive to antibiotics.
Clinical & Veterinary

Comparison of cgMLST Pipelines for Hospital Outbreak Detections

Comparison of cgMLST Pipelines for Hospital Outbreaks
Whole Genome Sequencing
03 Nov 2025 Lora Benoit Ph.D
Three commercial cgMLST pipelines were compared, using 255 hospital outbreak isolates, evaluating their concordance in identifying outbreak-related clusters and whether they consistently distinguish “clustered” vs “non-clustered” isolate pairs. 
Clinical & Veterinary

Phage Cocktail Tailored to Treat Hospital Acquired Enterobacter cloacae Complex Infections

Multi-phage Formulations Attack Enterobacter cloacae complex
ColiphagesEnterobacter Cloacae Complex (ECC)ESKAPE Pathogens
03 Nov 2025 Lora Benoit Ph.D
Study demonstrates that an institution-specific phage cocktail, built using local strain surveillance, can reduce time-to-effective therapy, curb transmission of resistant clones, and limit off-target ecological effects compared with broad, non-targeted antimicrobial agents.
Clinical & Veterinary

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