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Journal Club: assay-development

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Rapid Detection of Salmonella using a RPA–CRISPR/Cas12a Platform 

Salmonella enterica detection using a RPA CRISPRCas12a Platform
CRISPRLAMP/Isothermal AmplificationSalmonella
07 May 2026 Lora Benoit Ph.D
Novel diagnostic system for rapid detection of Salmonella enterica that combines isothermal nucleic acid amplification with CRISPR-based collateral cleavage to enable highly sensitive and specific detection.
Food & Beverage Clinical & Veterinary Assay Development

Journal Club - 27 April to 12 May 2026  

Journal Club
Antibiotic SensitivityArtifical IntelligenceBurkholderia cepacia complex (BCC)
12 May 2026 Lora Benoit Ph.D
Latest from our Journal Club: Variability in AST systems for Burkholderia cepacia complex, AI-integrated biosensors for rapid pathogen detection in complex food systems, CRISPR-based diagnostic platform enabling rapid, sensitive detection of Salmonella enterica.
Food & Beverage Clinical & Veterinary Assay Development

Journal Club - 2 April to 9 April 2026

Journal Club
Bacillus cereusProbioticsToxins
14 Apr 2026 Lora Benoit Ph.D
Latest from our Journal Club: B. longum ssp. infantis EVC001 reduces NEC in preterm infants, improved cereulide detection in powdered formula, and new models on how temperature, pH, and water activity drive cereulide production by Bacillus cereus.
Food & Beverage Clinical & Veterinary Microbiome Assay Development

Improved Extraction of Cereulide in Infant Formula for LC-MS/MS-Based Detection 

Improved recovery of cereulide from powdered infant formula and oil
Bacillus cereusToxins
09 Apr 2026 Lora Benoit Ph.D
Reliable detection of cereulide in powdered infant formula requires optimized water/acetonitrile extraction with salting-out, highlighting that extraction chemistry - not just LC-MS/MS instrumentation - is critical for accurate quantification in complex, lipid-rich matrices.
Food & Beverage Assay Development

Novel Test Strategy for Highly Pathogenic Avian Influenza A

Testing for Bird Flu using combined molecular approach
Assay DevelopmentAssay ValidationAvian Influenza
10 Jan 2026 Lora Benoit Ph.D
By combining FDA-authorized clinical and research-developed RT-PCR assays, researchers enabled adaptable, cost-effective diagnostics and large-scale surveillance for highly pathogenic avian influenza A H5.
Clinical & Veterinary Assay Development

New Enrichment Broth Finds B.cereus in Half of Powdered Infant Foods Sampled

Improved Recovery of B cereus From Infant Formulas
Assay ValidationBacillus cereusToxins
09 Jan 2026 Lora Benoit Ph.D
Using a novel enrichment (R & F® Bacillus cereus Group Enrichment Broth) and detection scheme, researchers found B.cereus group species in 50 per cent of PIF and infant cereal samples. WGS and cytotoxicity assays confirmed the presence of potentially virulent strains.
Food & Beverage Assay Development

BACTEC Validated for Sterility Testing of ATMP Suspensions

BACTEC can be used for sterility testing of ATMP Suspensions
ATMP - Advanced Therapeutic Medicinal ProductsCell and Gene TherapiesSterility Testing
02 Jan 2026 Lora Benoit Ph.D
The BACTEC automated microbial detection system reliably detects low-level contamination (≥ 5 CFU) in stem cell products suspended in Ringer’s Lactate, demonstrating full concordance with pharmacopeial sterility testing while delivering significantly faster results.
Assay Development

Shedding Light on 'Low Endotoxin Recovery' in LAL Assays

Low Endotoxin Recovery in LAL Assays
Clinical TrialsEndotoxinPyrogen Free Labware
04 Dec 2025 Lora Benoit Ph.D
Excipients used in pharmaceutical formulations can induce endotoxin masking, causing the Limulus Amebocyte Lysate (LAL) assay to underestimate true endotoxin levels. Gorman et al. show that masking arises from excipient-driven alterations in endotoxin structure and aggregate size.
Clinical & Veterinary Assay Development

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