Molecular Diagnostics – NGS, NA, Panels, POC

JAVASCRIPT DISABLED - This site works best with JavaScript enabled.

« Previous article
MWE Liquid Media &nd...

Next article »
Beckman Coulter&rsqu...

23rd April 2024 Content supplied by: various sources as indictated

Molecular Diagnostics - a rapidmicrobiology Special Focus

Molecular diagnostic tests can include techniques such as Polymerase Chain Reaction (PCR), real-time PCR, Whole-Genome Sequencing (WGS), Next Generation Sequencing (NGS), and Nucleic Acid Hybridization among others. Here we summarise the many advantages and some disadvantages of using these techniques in routine laboratories.

Advantages of Molecular Diagnostics in Clinical and Food Laboratories:

High Sensitivity and Specificity: Tests offer high sensitivity and specificity, delivering accurate detection of pathogens even when present at low concentrations.
Rapid Results: Molecular-based tests deliver faster results enabling more timely diagnosis and treatment in clinical settings and quicker identification of foodborne pathogens in food laboratories.
Multiplexing Capability: Many techniques allow for multiplexing, where multiple targets can be detected in a single test, saving time and resources.
Automation: Tests are often designed to be run on automated instruments, helping to address skilled staff shortages, reducing human error, and increasing efficiency.

Limitations of Molecular Diagnostics in Clinical and Food Laboratories:

Cost: Tests can be expensive, with kits tied into use only on instruments from a specific manufacturer, limiting choice.
Complexity: Specialized equipment and trained personnel can make them heavy on maintenance and training costs.
Interference: Factors like inhibitors in samples or contamination can affect the accuracy of molecular diagnostic results.
Limited Detection Range: Some MDx tests may have a limited detection range, missing certain pathogens or genetic variations.
Validation and Standardization: Ensuring the accuracy and reliability of molecular diagnostic tests requires rigorous validation and standardization processes.

In this rapidmicrobiology special focus, we showcase some options for laboratory-based molecular testing, including MWE's and Puritan's swab specimen collection and transport options ideally suited to molecular diagnostics, Himedia's HiGenoMB^® range is a complete portfolio of accurate and sensitive Real-time PCR Kits for clinical diagnostics and their AMR panel includes CE/IVD approved kits for the most prevalent drug resistance observed clinically in both gram-negative as well as gram-positive bacteria.

Tianlong’s Human Papillomavirus (HPV) Nucleic Acid Detection Kit is a qualitative in vitro test for detection of 18 high-risk HPV types. As an aid in diagnosing HBV infection and as a screening test for blood and plasma donors. Recently introduced, the BioPerfectus Survival motor neuron 1(SMN1) Gene Detection Real-Time PCR Kit is intended for the in-vitro qualitative detection of Survival motor neuron 1 (SMN1) exon 7 and(or) exon 8 deletions and the copy number of SMN1 at the 7th exon and (or) the 8th exon in human EDTA anticoagulated blood samples. Beckman Coulter’s validated HBsAg High Positive algorithm eliminates the need for repeat and confirmatory testing for samples ≥ 100.00 S/CO.

For food safety laboratories there is fully automated Suretect from Thermo Fisher, GENE-Up from bioMerieux and the more portable RiboFlow^®- Listeria Twin Detection Kit from SY-LAB.

For any approach, using the right standards and quality control protocols are key, Andrew Zenger, the Global Product Manager at ZeptoMetrix discusses why not relying on manufacturers-supplied QC standards are essential to achieving accurate results.

MWE Liquid Media – The Best Solutions for Molecular Diagnostics

WGS, NGS, Multiplex Panels, POC: Whatever the platform, Medical Wire have the best solutions for your specimens – and we can multi-task!
Find Out More

Think Outside the Vial with Swab Molecular Quality Controls

Zeptometrix^® NATtrol infectious disease molecular controls are now available in both liquid and swab format and can be used across various molecular testing platforms and assays.
Find Out More

GENE-UP^® NUTRAPLEX™ PRO - Molecular Diagnostics Innovation for Nutraceuticals

GENE-UP<sup>®</sup> NUTRAPLEX™ PRO - Molecular Diagnostics Innovation for Nutraceuticals

GENE-UP^® NUTRAPLEX™ PRO, by bioMérieux, is a cutting-edge molecular diagnostics innovation for the Nutraceutical Market. For the dietary supplement market, this multiplex assay detects Escherichia coli, Salmonella spp., and Staphylococcus aureus from a single universal enrichment in 24 hours.
Find Out More

HiMedia's Molecular Solutions for Detection of Antimicrobial Resistance

WHO has declared Antimicrobial Resistance as a serious global threat, killing millions of people worldwide. Additionally, the interdependency in the life cycle of humans, animals and environment have also played a cumulative role in the rise of AMR.
Find Out More

BioPerfectus Launches New Diagnostic Solution for SMA (Spinal Muscular Atrophy)

Bioperfectus introduce their Prenatal and Neonatal Care testing solution, which offers SMA (Spinal Muscular Atrophy) testing for expectant mothers and newborn screening, empowering and prioritizing the health of mothers and newborns.
Find Out More

Third-party Molecular Controls Are Crucial to Accurate Testing in Clinical Laboratories

In this interview with Andrew Zenger, the Global Product Manager at ZeptoMetrix, we learn about how third-party molecular controls are key to developing clinical lab quality practices, regulatory compliance, and factors that labs should consider when choosing a supplier of third-party QC materials.
Find Out More

Beckman Coulter’s New CE-Marked Hepatitis Assays at ESCMID Global

Beckman Coulter Diagnostics has extended the menu of DxI 9000 Immunoassay Analyzer assays. Recently, tests for Hepatitis B and C viruses received CE marks. These new tests will be presented at ESCMID Global, the largest European congress in the fields of clinical microbiology and infection.
Find Out More

RiboFlow^® Listeria Twin - Rapid and Simple Molecular Detection

RiboFlow<sup>®</sup> Listeria Twin - Rapid and Simple Molecular Detection

The RiboFlow^®- Listeria Twin Detection Kit enables highly specific yet very simple detection of Listeria monocytogenes and other Listeria species within just a few minutes, with little effort and equipment.
Find Out More

Copan at ECCMID 2024

Collection, processing, and diagnosis: Copan’s 2024 ECCMID will be dense with great science and exciting events. Discover more.
Find Out More

Visit Puritan^® at ECCMID, Booth #E-61 — See You There

Visit Puritan<sup>®</sup> at ECCMID, Booth #E-61 — See You There

Learn about Puritan^® Medical Products Company and their extensive line of specimen collection and transport products as well as their Custom Product Manufacturing capabilities at ECCMID, Booth E-61 in Barcelona, Spain, April 27th – 30th, 2024.
Find Out More

Tianlong Human Papilloma Virus (HPV) PCR Kit Detects 18 High Risk HPV Types

Tianlong’s Human Papillomavirus (HPV) Nucleic Acid Detection Kit is a qualitative in vitro test for the detection of 18 high-risk HPV types, which can specifically identify types of HPV 16 and HPV 18 while concurrently detecting the remaining other high-risk types.
Find Out More

Enhance the Efficiency and Productivity of Your PCR Food Safety Testing

With the new Thermo Scientific™ SureTect™ Automation Workflow, you can take your food safety and quality testing to the next level with automated lysis and PCR setup.
Find Out More

Achieve Precision Quantification in Every Reaction With qPCRBIO SyGreen^® Mixes

Achieve Precision Quantification in Every Reaction With qPCRBIO SyGreen<sup>®</sup> Mixes

Get unparalleled results in your microbiological research. qPCRBIO SyGreen^® Mixes are developed for reliable gene expression analysis, in 1- or 2-step RT-qPCR, accurate species abundance quantification in complex samples, and use in any other application of dye-based qPCR. Read on to learn more.
Find Out More

A New Milestone in Quality Control for Novogene's 16S Amplicon Metagenomic Sequencing

Novogene introduces sample internal quality control management tools in the amplicon experiment process. It ensures real-time data monitoring, contamination source identification, and data quality assurance, providing the highest quality and reliable amplicon sequencing and data analysis services.
Find Out More

Innovative New SmartLid™ The Smarter Way for Rapid Viral DNA/RNA Extraction

Now available from Alpha Laboratories Ltd, SmartLid™ offers a smarter way to handle nucleic acids in molecular biology applications. Delivering high yields of ultra-pure DNA/RNA for sensitive downstream processing SmartLid sets a new standard for speed in nucleic acid extraction efficiency.
Find Out More

Share on:

Tags: