Mycoplasma contamination is one of the most pervasive and underestimated challenges in cell culture. These wall-less, ultra-small bacteria often evade traditional detection methods—such as microscopic inspection or standard culture techniques—due to their minimal size and slow growth. Yet, their presence can have a profound impact: altering cell metabolism, triggering chromosomal aberrations, modifying gene expression, and compromising experimental reproducibility. Because they often persist silently, undetected mycoplasma can derail entire studies, skew critical data, and threaten product safety in biomanufacturing. Rapid and reliable detection is therefore not just good laboratory practice—it’s essential to research integrity and scientific credibility.
Now, researchers can identify even low-level contamination quickly, accurately, and confidently using real-time PCR (qPCR) technology. The MycoDetect qPCR Detection Kit from Microzone is your go-to solution for the sensitive detection of Mycoplasma DNA in cell culture samples. Targeting the highly conserved 16S-rDNA region, the kit identifies a wide spectrum of mycoplasma species including Mycoplasma arginini, Mycoplasma fermentans, Metamycoplasma hominis, Mesomycoplasma hyorhinis, Metamycoplasma orale, Mycoplasmoides pneumoniae, Acholeplasma laidlawii and others commonly associated with cell culture contamination. Designed for rapid turnaround and ease of use, MycoDetect delivers definitive results in an hour—empowering your lab to detect contamination before it compromises your research.
The kit utilises dual-labelled hydrolysis probe technology, which ensures high specificity and sensitivity with minimal cross-reactivity. The assay includes an Internal Amplification Control (IAC) to verify PCR validity, ruling out false negatives and ensuring full confidence in your results. Additionally, the included MegaMix Platinum qPCR Mastermix contains dUTP/UNG chemistry, which actively eliminates false positives caused by amplicon contamination, further enhancing the reliability of your data.
Key Features:
- Detects a broad range of mycoplasma species via 16S-rDNA targeting
- Confirms run validity with built-in Internal Amplification Control (IAC)
- Maximises accuracy using dual-labelled hydrolysis probe technology
- Delivers results in 1 hour with rapid, streamlined protocol
- Supports both extracted DNA and direct-to-PCR lysates
- Integrates easily with standard qPCR instruments (FAM and HEX channels)
- Eliminates setup hassle with fully optimised, ready-to-use reagents
What’s Included:
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2X MegaMix Platinum qPCR Mastermix (with dUTP/UNG)
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Mycoplasma Detection Mix
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Positive Control DNA
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Just Water (Negative Control)
Whether you're safeguarding cell line purity, monitoring production runs, or verifying biopharma workflows, the MycoDetect Kit provides speed, sensitivity, and confidence. Don’t let silent contamination disrupt your work - order MycoDetect today.
