Merlin MICRONAUT System - Direct Susceptibility from Positive Blood Cultures

The procedure of direct susceptibility testing of pathogens from positive blood cultures with the MICRONAUT system was evaluated externally by using the culture flasks PLUS Aerobic/F, PLUS Anaerobic/F, and PLUS Pediatric.

For direct susceptibility testing with the MICRONAUT system, 8 ml of the positive blood culture medium is centrifuged at 800 rpm for 10 min.

The supernatant is transferred into a new tube and centrifuged at 3,000 rpm for 5 min. and the resulting pellet is diluted in sterile 0.9% saline to prepare a 0.5-McFarland-standard suspension.

This suspension is then inoculated according to the instructions for the MICRONAUT system for antimicrobial susceptibility testing.

Reference:
Evaluation of the Merlin MICRONAUT System for Rapid Direct Susceptibility Testing of Gram-Positive Cocci and Gram-Negative Bacilli from Positive Blood Cultures.

Nele Wellinghausen et al., Journal of Clinical Microbiology 45: 789 -795 (2007)