Bio-Rad Laboratories

« Previous article
Why Bio-Rad? Learn M...

15th January 2018  Content supplied by: Bio-Rad Laboratories

Bio-Rad RAPID'Campylobacter Media Fits New European Regulation

Campylobacter is a bacterium that can cause an illness called campylobacteriosis in humans. With over 190,000 human cases annually, this disease is the most frequently reported food-borne illness in the European Union (EU). However, the actual number of cases is believed to be around nine million each year. The cost of campylobacteriosis to public health systems and to lost productivity in the EU is estimated by EFSA to be around EUR 2.4 billion a year.

To protect consumers from this public health threat, the EU has adopted an integrated approach to food safety from the farm to the fork.

Campylobacteriosis is a zoonosis, a disease or infection that can be transmitted directly or indirectly between animals and humans. Usual symptoms are fever, diarrhea and abdominal cramps. Raw poultry meat is often contaminated with Campylobacter since the bacterium can live in the intestines of healthy birds. It is also found in pigs and cattle. Eating undercooked chicken, or ready-to-eat foods that have been in contact with raw chicken, is the most common source of infection.

Considering all this, a European commission regulation has been just adopted to include the food category "Carcasses of broilers" for the control of Campylobacter using the analytical reference method (EN ISO 10272-2).

The regulation recommends application of International Standard EN ISO 10272-2, which defines the horizontal method for enumeration of Campylobacter in food and feed stuffs as a reference method for verifying compliance with the criterion for Campylobacter in poultry carcasses for the official laboratories. Regulation allows also using alternative method ISO 16140 validated.

ISO 10272 indicates the use of a charcoal-based selective media or a blood containing media. These culture plates are incubated for up to 72 hr and their lack of selectivity allows for the growth of large amounts of competing flora. In order to help customers, Bio-Rad has developed a specific chromogenic media to enumerate easily Campylobacter.

RAPID'Campylobacter agar is a selective chromogenic media for the detection and enumeration of the main species of thermophilic Campylobacter (C. jejuni, C. coli and C. lari) in food products and environmental samples.  The use of a selected nutritive mixture associated with reducing agents allows the growth of Campylobacter spp. in an optimal time (24-48 hr). After incubation, the plates can be stored refrigerated (2-8°C) for 72 hr in a microaerophilic atmosphere before reading. Other bacterial species, as well as yeasts and molds, are inhibited by the selective agents. Campylobacter produce brick-red colonies on RAPID’Campylobacter.  A confirmation method has also been developed after 24 hr with PCR or after 48 hr with a latex test.

The RAPID'Campylobacter method for enumeration was validated according to the EN ISO 16140 protocol and the AFNOR technical rules (Certificate number 07/25 - 01/14). Bio-Rad has started to renew the validation according to the new ISO 16140-2 standard.

During a study performed by University of Ghent in 2014 to compare different media, results clearly indicated that RAPID'Campylobacter is highly selective without loss of sensitivity for recovering Campylobacter. Results obtained are in agreement with those for other commonly used media; therefore, RAPID'Campylobacter is suitable for Campylobacter enumeration in poultry meat samples. (Click here to download the study).

For more information, visit:

Share on:


Date Published: 15th January 2018

Source article link: View

View full company details

Related news