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2nd April 2024  Content supplied by: Condalab

The Perfect Media for Your Cultures With Recombinant E. coli

Escherichia coli is an Enterobacteriaceae, typically found in the intestinal microbiota of animals such as humans. It was one of the first bacteria to be studied and sequenced and then became the first option for the biotech industry. Its popularity comes from the following factors.

  • There is a wealth of knowledge about its metabolism and biochemical pathways
  • It is compatible with a large number of molecular tools
  • It is easy to work in the laboratory
  • It has a high growth rate, generating a high bacterial density in a short time.

The number and types of recombinant proteins that can be obtained through E. coli is wide: medications, active enzymes, food additives, biofertilizers... In order to have optimal yields, the culture medium is essential; one of the most efficient strategies is the use of Luria-Bertani (LB Lennox) culture broth and Terrific broth.

LB Lennox broth is ideal for generating rapid growth from a low concentration of bacteria, even after undergoing aggressive processes such as thawing. It is the preferred culture medium to achieve the necessary density of the inoculum that will be taken to the bioreactor. It is made up of a hydrolysate mixture that offers all the essential nutrients.

On the other hand, Terrific broth is a very rich medium, optimized specifically for the growth of E. coli and the synthesis of recombinant molecules. Its composition is based on a higher concentration of hydrolysates; with a contribution of glycerol as a carbon source and phosphates that provide essential elements such as phosphorus or potassium.

If you want to know more about these two products, do not hesitate to visit the Condalab website, where we will help you optimize your process.

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Date Published: 2nd April 2024

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