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23rd May 2016  Content supplied by: Promicol B.V.

ATP Bioluminescence Rapid Testing of UHT/ESL Dairy Products

The Promicol system provides a rapid and reliable method for testing microbial ATP content in UHT and ESL Dairy products. It is currently used by many international companies to routinely release finished products. The 2 days pre-incubation time at 30 °C (UHT) or 20 °C (ESL) provides companies a saving of at least four days over the current traditional test methods for release of their products.

The test procedures are simple, do not require any sample preparation and are very easy to use. The Promicol system can process and automatically analyze the data from approximately 200 samples per hour, offering significant reductions in labor costs.

The Promicol system and microbial ATP test kit for Dairy UHT/ ESL products can test a wide range of Dairy and Dairy based UHT/ ESL products. Some examples are listed below:

  • White milks
  • Skim milks
  • Milks with supplements
  • Milk shakes
  • Chocolate milks
  • Flavored milks
  • Lactose reduced milks
  • Puddings
  • Infant formulae
  • Creams & creamers
  • Soya beverages
  • Cat milks
  • Cooking sauces
  • Non-dairy creamers
  • Cereal beverages
  • Puppy milks
  • Desserts
  • Custards

ATP Testing


For testing, the pre-incubated packages need to be shaken properly before sampling to ensure a homogenous sample. 50 µl aliquots of each product are placed in a microplate well and the Promilite M4 instrument performs the assay automatically by using the implemented ‘UHT/ ESL‘ protocol which is described below: First samples are treated with an apyrase reagent for 15 minutes to remove free ATP from the samples. Afterwards a microbial extractant is added in order to release any microbial ATP from microorganisms present in the sample. Finally the firefly enzyme is used to turn the released microbial ATP into light. This is measured by the Promilite M4 instrument and expressed in Relative Light Units (RLU). The software automatically classifies the measured values as pass, re-test or fail and therefore enables an easy interpretation for the user.

Promicol aims to develop a long term relationship with its customers, built on trust, innovation and expertise. It is our goal to provide a service that is in touch with the needs of your quality management and to your demands from rapidly changing markets, making ATP work for you, together with you.

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Date Published: 23rd May 2016

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