Bio-Rad Laboratories

19th September 2022  Content supplied by: Bio-Rad Laboratories

Bio-Rad’s AFNOR Validations for New Legionella PCR Protocols

Bio-Rad has received AFNOR validation for the iQ-Check Legionella Aquadien DNA extraction short protocols including the addition of the Free DNA Removal Solution protocol according to ISO TS 12869 and NF T90-471.

These validations allow Bio-Rad to be the first and only Legionella PCR method validated by a third party that includes management of free DNA in the sample.

Bio-Rad provides a complete range of products for the detection and quantification of Legionella. Products comply with regulatory standards and allow for risk management based on accurate and rapid results. These Legionella testing products are suitable for water supply systems, water intended for human consumption, hot water for sanitation purposes, water for industrial use, natural mineral water, water for recreational activities, biofilms, and aerosol samples. 

PCR range includes iQ-Check Legionella spp. and L. pneumophilia kits, associated with the high-performance Aquadien DNA Extraction kit

  • Compatible with all types and volume of water (water systems, drinking water as well as cooling tower water)
  • Results delivered in 4 hours from sample receipt with no confirmation needed
  • Can detect and quantify Legionella spp. and L. pneumophilia in the same assay
  • 96 well format allows for an adaptable number of assays per run
  • Fully automated results with internal positive and negative control
  • Free DNA Removal Solution option to improve the reliability of results
  • AFNOR VALIDATION certified according to NF T90-471 and ISO TS 12869
  • Reliable for colony confirmation according to NF T90-431 and NF EN ISO 11131

Watch Bio-Rad's Recorded Webinar on Legionella Testing Solutions and learn more at

Important information from the European Parliament:
Flag of the European Parliament
On December 16, 2020, the European Parliament formally adopted the revised Drinking Water Directive, (the quality of water intended for human consumption) with a focus on Legionella testing.  PCR is clearly identified as a complementary tool for Legionella testing when the method is validated against the ISO TS 12869 standard.


To learn more click on the green "Request Information" button below.

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Date Published: 19th September 2022

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