Join Dr. Kara Held, Baker Science Director, for this information session on volatiles and Class II A2 BSC.

• Volatile chemicals, while typically used in a fume hood, sometimes need to be used in conjunction with biological experiments, requiring the use of volatile chemicals within a biosafety cabinet (BSC).  
• While the safest option often mentioned is a 100% exhausted Class II Type B2 cabinet, this is not always possible.  
• International safety standards have recommended “minute” amounts of volatiles can safely be used in a Class II Type A2, but what constitutes “minute” can be left up for interpretation.  
• We have derived an equation to calculate the internal concentration, as well as the time required for the evacuation of any volatile chemical, shown both by calculation and experimentation. 

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