NAT-MAT® is a next-generation monocyte activation test (MAT) based on digital PCR that enables the detection of pyrogen contamination in pharmaceuticals according to Ph. Eur. 2.6.30, and thus can replace the Rabbit Pyrogen Test. The NAT-MAT® relies on the use of a NAT-MAT Cells® (monocytic cell line) which are stimulated with a pyrogen standard and the sample to be tested followed by nucleic acid extraction and dPCR analysis of the gene expression of IL-1β and TNF-α.
Key Points:
- Ph. Eur. 2.6.30 compliant
- Highly automated workflow
- Rapid cell stimulation (only 4 hours)
- Normalizing pyrogen concentration to cell number
- Data analysis with easy-to-use software
