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17th November 2021  Content supplied by: BIOTECON Diagnostics GmbH

PCR Found to be the Better Method for Legionella Colony Confirmation


Researchers from Industrielle Werke Basel (IWB) and Kantonales Labor Zürich recently published a study that found PCR to be the ideal tool for confirming Legionella colonies.

In the comparison study, not only was the PCR-based method found to be very quick but colonies were also identified with the highest level of specificity. Furthermore, cross-reactions leading to false positives or false negatives did not occur with our proven test. This was all verified by MALDI-TOF performed in the Swiss reference laboratory.

BIOTECON Diagnostics’s microproof®Legionella Quantification LyoKit is set up as a multiplex test, identifying or even quantifying L. spp., L. pneumophila, and L. pn. serogroup 1 in one run.

Documented limitations of agglutination-based analysis do not occur with our proven test. This ISO 11731 compliant method will set the benchmark for all laboratories worldwide that still carry out time-consuming sub-cultivations followed by agglutination tests with limited specificity.

The study entitled, Comparison of the culture method with multiplex PCR for the confirmation of Legionella spp. and Legionella pneumophila is an open-access study and can be viewed here

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Date Published: 17th November 2021

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