- New Lyo-Ready Inhibitor-Tolerant reagents enable developers to build highly stable, ambient-temperature assays without compromising performance in challenging sample types such as blood, saliva, and other inhibitor-rich matrices.
- Reagents are in a lyophilisation-ready format already been widely adopted for high-performance PCR and qPCR in complex biological samples.
- By integrating this chemistry into a lyo-ready 4x master mix, the company now provides diagnostic developers with reagents that withstand inhibitors and retain room-temperature stability after drying.
- The result is a flexible reagent platform that simplifies upstream sample preparation and supports dependable amplification even when sample extraction is limited, ideal for real-world diagnostics using blood and saliva samples which contain compounds known to hinder qPCR and RT-qPCR reactions.
- Resisting these inhibitors, helps minimise false negatives, preserve sensitivity, and improve assay reliability in direct or minimally processed workflows.
PCR Biosystems has announced the launch of its new Lyo-Ready Inhibitor-Tolerant reagents, bringing the company’s powerful inhibitor-tolerant chemistry into its growing range of lyophilisation-ready reagents for molecular diagnostics.
This new product line enables developers to build highly stable, ambient-temperature assays without compromising performance in challenging sample types such as blood, saliva, and other inhibitor-rich matrices.
Inhibitor-tolerant performance, now in a lyophilisation-ready format
PCR Biosystems’ inhibitor-tolerant chemistry has already been widely adopted for high-performance PCR and qPCR in complex biological samples. By integrating this chemistry into a lyo-ready 4x master mix, the company now provides diagnostic developers with reagents that withstand inhibitors and support room temperature stability after drying.
The result is a flexible reagent platform that simplifies upstream sample preparation and supports dependable amplification even when sample extraction is limited.
Ideal for real-world diagnostics using blood and saliva samples
Blood and saliva are increasingly being used in non-invasive diagnostic workflows, but both contain compounds known to hinder qPCR and RT-qPCR reactions. By resisting these inhibitors, the Lyo-Ready mixes help minimise false negatives, preserve sensitivity, and improve assay reliability in direct or minimally processed workflows. This makes the mixes particularly valuable for decentralised testing environments, high-throughput public health programmes, and point-of-care applications.
Designed for ambient storage, simplified logistics and global reach
As fully lyo-ready, glycerol-free formulations, the new mixes are engineered for drying without additional optimisation. Once lyophilised, the reagents can be shipped and stored at ambient temperatures, removing the need for cold-chain infrastructure and reducing logistical complexity across global supply routes. This ambient stability enables diagnostic developers to create dried assay kits that are well suited for resource limited laboratories, decentralised testing settings, and workflows where simplified storage and transport are essential.
Two formats for complete DNA and RNA detection workflows
The product range includes:
- Lyo-Ready Inhibitor-Tolerant Probe Mix - For high-sensitivity DNA detection in probe-based qPCR.
- Lyo-Ready Inhibitor-Tolerant Probe 1-Step Kit - A complete 1-step RT-qPCR solution for RNA detection, featuring a high-concentration UltraScript® reverse transcriptase and RNase inhibitor for strong performance in demanding assays.
Both mixes are 4× concentration, glycerol-free, and pre-blended with lyophilisation-compatible excipients, ensuring high activity after resuspension and reliable performance across diverse assay formats.
Built for singleplex and multiplex testing
Whether developing a targeted single-gene assay or a multi-pathogen diagnostic panel, the new mixes deliver strong, reproducible amplification with excellent probe compatibility. Their tolerance to inhibitors paired with lyophilisation-readiness makes them highly suited to multiplexed diagnostic tests intended for broad, real-world deployment.
“Achieving consistent results from complex biological samples remains a major challenge for assay
developers,” said Alex Wilson, Co-Founder at PCR Biosystems. “Our new Lyo-Ready Inhibitor-Tolerant reagents bring together inhibitor tolerance and lyo-ready stability to deliver high-sensitivity qPCR and RTqPCR across a wide range of challenging workflows, without compromising performance.”
The Lyo-Ready Inhibitor-Tolerant Probe Mix and Probe 1-Step Kit are now available for quotes and sample evaluation. PCR Biosystems offers full technical support, custom formats, bulk supply and OEM manufacturing options for diagnostic developers.
