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20th April 2021  Content supplied by: SY-LAB Geraete GmbH

New Rapid Miniaturised MPN Method for Butyric Acid-Forming Clostridia Testing


SY-LAB has recently introduced a new technology for the detection of butyric acid-forming (cheese-spoiling) clostridia, based on the use of a miniaturized MPN method. This new technology incorporates the following methodical highlights:

  • Chromogenic detection principle: A chromogenic substrate is used, which indicates the growth of the cheese spoiling clostridia by a color change from red to yellow. Automated evaluation is possible using a scanner system with image and data processing (AMP-6000® - automated MPN platform)
  • Selectivity: By using specific inhibitors, the competitive microflora is prevented from growing, thus ensuring selective detection of the cheese spoiling clostridia.
  • Use of a nutrient medium concentrate: The specific nutrient medium (AmpMedia 666) is offered as a ready-to-use 4-fold concentrate using the test sample (milk) as diluent. As a result, detection levels as low as 20 spores per liter can easily be achieved.
  • Analysis time: The new process delivers results in as little as 48 hours and is therefore up to 5 days faster than traditional methods.
  • Precision: Based on the miniaturization of the method, significantly more sample replicates can be analyzed in the MPN approach. This will increase the accuracy of the result by a multiple.
  • Automation: Due to the possibility of automatic evaluation via the color change and result calculation, the new method is automatable. Different robotic solutions for automaton of the testing workflow are available.

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Date Published: 20th April 2021

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