10th February 2022 Content supplied by: NimaGen
EasySeq™ RC-PCR SARS-CoV-2: Easy, Fast and Accurate Outbreak and Variant Analysis
EasySeq™ SARS-CoV-2 helps laboratories generate whole genome sequencing data from cDNA, derived from COVID-19 positive tested patients. Too date, EasySeq™ RC-PCR SARS-CoV-2 has been a significant contributor to fighting COVID-19, facilitating the rapid and accurate tracking of newly emerging variants of the coronavirus, such as Delta and Omicron variants, including Omicron subvariant BA.2
With the NimaGen EasySeq™ RC-PCR Library Preparation method, the implementation of an NGS driven viral surveillance strategy could not be simpler or safer. Utilising patented Reverse Compliment (RC-PCR) technology, sequence ready libraries are generated from cDNA covering the entire 29.9Kb SARS-CoV-2 Genome with minimal hands-on time and just a single PCR tube for both amplification and indexing, which reduces the risk of error and contamination.
Tiled RC-PCR primers and probes are provided in tubes, as well as 2 breakable 96 well plates which include UDI Indexes. Just add cDNA and the RC-PCR mastermix provided in the kit and load on to your PCR instrument. Post-PCR all samples can be combined for pooled cleanup with the included Ampliclean Magnetic Bead Cleanup Kit and following standard quantification and QC checks your libraries are ready to sequence. This workflow solution is ideal for low- to high-throughput automation.
NimaGen developed a dedicated GISAID compatible software tool for analyzing NGS data from the SARS-CoV-2 virus. This software is provided for free, with all needed settings ready installed, when purchasing a starter package for 96 samples.
Please contact NimaGen or click on the Request Information button below.
Date Published: 10th February 2022
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