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1st August 2016  Content supplied by: SeraCare Life Sciences, Inc.

AccuPlex™ Recombination Virus Technology-based Zika Reference Material

SeraCare Life Science has launched AccuPlex Zika Reference Material to fast track the development and validation of PCR-based Zika virus assays. Based on SeraCare’s patent-pending AccuPlex recombinant virus technology, AccuPlex Zika Reference Material provides a safe, non-replicative, and fully-extractable positive material which covers the entire Zika virus genome.

Utilizing AccuPlex recombinant virus technology, SeraCare’s Zika reference material contains the entire RNA genome of the ZIKV 2007 strain in four separate overlapping virus constructs. This product is formulated at ~1.0E+05 copies/ml in 0.25ml of defibrinated human plasma; the copy number is assigned based on results from a digital PCR assay developed in-house.As a recombinant virus product, AccuPlex Zika Reference Material utilizes a mammalian RNA virus that closely mimics the Zika virus in both size and complexity. Ongoing accelerated and real-time stability studies indicate that AccuPlex recombinant viruses are stable at room temperature for at least 20 months and at 4 ºC for at least two years. “We have developed and manufactured this plasma-based product in just a matter of weeks by leveraging our scalable, recombinant virus technology,” says Dr. Bharathi Anekella, Vice President of Research and Development at SeraCare Life Sciences. “As a result, we have provided assay developers, such as Siemens Healthineers, a reliable source of safe, positive material to aid in the development of their Zika virus assays.” Siemens developed their PCR assay (RUO) to test for the presence of the Zika virus prior to the development of antibodies.

In addition to assay development, AccuPlex Zika Reference Material is an ideal product for public health agencies around the globe to implement training, interlab studies, and proficiency programs. As a safe, non-infectious, positive reference material, this product is part of an ever growing portfolio of critically important products from SeraCare for better validation and characterization of emerging molecular assays.



Date Published: 1st August 2016

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