The RiboFlow® Listeria Twin Detection Kit enables highly specific, yet very simple detection of Listeria monocytogenes and other Listeria species within just a few minutes, with little effort and equipment.

A ribosomal RNA (rRNA) sequence specific for Listeria monocytogenes and a second rRNA sequence specific for all Listeria species are detected simultaneously by a proprietary nucleic-acid hybridisation protocol, in a simple lateral flow assay format, within 25 minutes. Tedious nucleic acid purification or enzymatic amplification of target sequence is not necessary.

Using that approach a dual result from one test-run can be obtained. Along with information on the absence of Listeria monocytogenes (ISO 16140-2 validated protocol), the test gives also an indication about presence or absence of Listeria spp.

Test kits come in a convenient box, with very small footprint, containing all reagents ready-to-use. The kits have a long shelf-life and can be stored at ambient temperature. The devices can also be used to confirm single suspicious colonies from any selective or non-selective agar plate and could be an additional tool for rapid molecular confirmation.