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28th November 2023  Content supplied by: Merck

Easy Non-PCR Molecular Screening for Contaminant Microorganisms

Conventional cultivation-based microbiological methods are time consuming and can take from 2 to 15 days to get results. Although rapid methods such as PCR are much faster, they usually require expensive instrumentation and equipment. Not so the HybriScan® molecular assays.

Our HybriScan® system provides fast and accurate qualitative or quantitative results for a wide spectrum of spoilage organisms and pathogens. It detects attomoles of microbe-specific rRNA using sandwich hybridization. A labelled capture probe is used to immobilize the target sequence on a solid support plate. The digoxigenin-labeled detection probe then provides an enzyme-linked optical signal read-out. Detection of the target results from applying anti-DIG-horseradish peroxidase Fab fragments. The bound complex is visualized by adding horseradish peroxidase substrate. This results is a yellow color that can also be measured at 450 nm using a microplate reader.

Results are available after only about 2 hours of testing. Pre-enrichment can improve sensitivity down to 1 CFU/L. Using the two different specific probes for detection of microbial rRNA minimizes the likelihood of a false positive result.

The main features of HybriScan® molecular screening system:

  • Quantitative or qualitative results by organism group or specific species (HybriScan®D kits for quantitative, HybriScan®I kits for qualitative testing)
  • Detects living cells only
  • Also detects non-culturable microbes
  • High specificity
  • Not sensitive to sample matrix
  • Cost-efficient read-out using a standard plate reader (or visually for qualitative results)
  • Results in about 2 hours
  • High sample throughput

The microorganisms to test water for include:

  • Escherichia coli
  • Legionella (in wastewater)
    Total Bacterial Count
    Wastewater Total Bacterial Count
  • Legionella pneumophila
  • Microthrix parvicella

Due to its insensitivity to sample matrices, the HybriScan® system can also be used to test a wide range of beverages for contaminants, including:

  • Typical beer-spoiling organisms
  • Various yeasts
  • Various lactobacilli
  • Alicyclobacillus and Pectinatus frisingensis
    Megasphaera cerevisiae
    Pediococcus damnosus
  • Brettanomyces
  • Leuconostoc
  • Pectinatus cerevisiiphilus

Visit Merck/Millipore Sigma for more or use the Request Information button below to connect directly with the supplier.

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Date Published: 28th November 2023

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