Millipore Sigma Webinar

Sigma-Swabs® Great stains - no haze!

Gram Stain from Sigma-swab
S. aureus recovered using dry Sigma-Swab (MWE)
Since the earliest days of microbiology, gram staining has been the starting point for many bacteriological investigations. Whether at the doctor's office for an initial presumptive characterisation based on stain and morphology, or at the laboratory bench as the starting point for a complete description of the prevalent organism.

This has carried on into the era of transport swabs, and even charcoal medium offers little interference with Gram stains. It has been reported, however, that some newer liquid swab based transport swabs with nylon flocked swab applicators release substances into the stain that cause interference with the staining process.
A study has been presented at this month's ASM General Meeting in San Diego1 that shows while Sigma-Swabs® (Dry swabs) and Sigma-Transwabs® (with liquid Amies medium) give clear Gram stains with no interfering background, both the E-swab transport swab (with liquid Amies medium), and its component dry flocked swab have a hazy background.

Further testing with acridine orange stain showed interference suggestive of possible proteinaceous additives.
Gram Stain from dry flock swab.
E.coli recovered
using dry flock swab from E-swab pack
Gram Stain from E-swab with Liquid medium.
S. aureus recovered
using E-swab with liquid medium

Sigma-Swabs® and Sigma-Transwabs® have a swab bud made from open celled polyurethane foam (Macrofoam™) with no additives and no non-viables for top quality stains without interference.

For great Gram stains without haze - choose Sigma-Swabs® and Sigma-Transwabs®

1. Stuczen, M., & Edwards-Jones, V., 2010, Comparison of the quality of Gram stain prepared using different swab transport systems, 110th General Meeting of American Society of Microbiology, San Diego.

NOTE: This item is from our 'historic' database and may contain information which is not up to date.

Source : Medical Wire & Equipment Co View Company Information

Posted on June 1, 2010

Fijifilm Pyrostar testing critical water used in the reprocessing of medical devices

SGL Plates and Culture Media for Pharmaceutical Microbiology

AI for microbiology

QC Standards Controls and Proficiency Testing Schemes

Get our eNewsletter
Over 7,000 microbiology professionals get our weekly eNewsletter - subscribe now and find out why!