Simplified SAME-DAY STEC Detection of Multiple Strains of E. coli in Ground Beef
|Life Technologies Corporation has developed a complete rapid molecular testing workflow for multiple strains of pathogenic Shiga-toxin producing Escherichia coli (STEC), a bacteria found in undercooked beef.
| Meat producers require a faster and simpler method to accurately detect multiple STEC strains, which can include the well-known O157:H7 serotype as well as more than 100 non-O157 strains.
The U.S. Department of Agriculture (USDA) now requires testing for the 'Big Six' non-O157 strains (O26, O45, O103, O111, O121 and O145) that cause about 70 percent of non-O157 infection, but this testing requires new analytical methods.
The USDA Microbiology Laboratory Guidebook (MLG) method is the formal approach recommended by the USDA's Food Safety and Inspection Service (FSIS) for the testing and isolation of the 'Big 6' STECs in ground beef and beef trim in the U.S. Life Technologies offers many of the reagents validated in the USDA FSIS MLG method.
While the MLG method provides confident results and isolation of the pathogen, the six -day process is too long, requires too many reagents, and requires significant technician training and processing, all of which drives up the cost.
Life Technologies has been collaborating with the USDA Agricultural Research Service (ARS) to validate assay designs against an extensive panel of both pathogenic and non-pathogenic STECs of various o- group and origin. The result is highly-specific, single-target PCR assays which can be multiplexed to provide high levels of accuracy for a high-throughput screening assay.
The Life Technologies workflow will provide faster, simpler detection with the same level of sensitivity and accuracy produced by the MLG method:
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Source : Life Technologies View Company Information
Posted on August 6, 2012