World's First ELISA-based Endotoxin Detection Method
Hyglos presents EndoLISA®, the world´s first commercially available ELISA-based endotoxin detection method.
EndoLISA® is the result of several years of research and test development aimed at establishing a better performing alternative to LAL. Hyglos has developed and optimized a lipopolysaccharide (LPS) specific phage-derived protein as well as optimal sample conditions, in order to specifically cover the entire substance group of LPS (endotoxin). The phage protein is pre-coated to the wells in a microtiter plate and as the sample is added to the well, the endotoxin (LPS) in the sample is bound to the phage protein. Any sample matrix with potentially interfering components is then completely removed by a washing step. Therefore, the subsequent detection by recombinant Factor C and a fluorescence substrate is thus left unaffected by inhibitors, facilitating a reliable quantification of endotoxin in the sample.
EndoLISA® at a glance:
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Source : Hyglos GmbH View Company Information
Posted on September 13, 2011