Merck Extends Product Range of Real-time PCR Assays
Since January 2008, Merck Millipore has distributed the innovative foodproof® real-time PCR products from Biotecon Diagnostics GmbH, Germany. These products had been previously distributed by Roche Diagnostics and are designed for use on Roche's LightCycler® instruments 1.x or 2.0.
Now, Merck Millipore has extended this range of foodproof® products with new real-time PCR kits which can be used on many other instruments such as TaqMan®-based instruments or Roche's LightCycler® 480. All these new products are marketed by Merck Millipore and their subsidiaries and dealers in countries worldwide.
The following foodproof® kits are now available for use on TaqMan-based instruments:
For Roche's LightCycler® 480, the following kits are now newly available:
The foodproof® product range are all 'real-time' PCR (RT-PCR) kits, a newer variation of the PCR technique, suitable for the detection of bacteria, viruses and other organisms, and allows the 'online' monitoring of the reaction process during the amplification process. This is a major advantage over 'standard' PCR kits which require laborious and time-consuming post-PCR steps such as gel electrophoresis to analyse the reactions.
In order to achieve valid PCR results, correct sample preparation which eliminates substances inhibitory to the Taq Polymerase enzyme is necessary. For all foodproof® RT-PCR kits, quick and easy-to use sample preparation kits are available. For most pathogens assays, short sample preparation kits (foodproof® ShortPrep I or II, or III kits) can be used. Using these kits, PCR assay time is reduced to only 30 minutes with less handling steps so that the overall testing time takes only about 1 to 1.5 hours. To detect the presence of bacteria, an enrichment time of 18 - 24 h is required, thus the time to result is shortened by several days compared to standard methods.
foodproof® real-time PCR products have the following benefits:
The foodproof® product line extends Merck's unique and innovative range for rapid and convenient automated or manual detection and confirmation of important food pathogens, such as Salmonella, Listeria spp., Listeria monocytogenes, E. coli O157, pathogenic Campylobacter, and other pathogens. All these rapid tests fit perfectly to Merck's comprehensive range of high-quality granulated dehydrated culture media.
For further information about the wide range of products available from Merck Millipore / EMD Chemicals for the growth, detection, isolation and identification of food- and waterborne pathogens and other microorganisms, please contact your local Merck organization or EMD Chemicals [now Millipore Sigma] (for USA and Canada).
Products for TaqMan-based instruments
Products for LightCycler 480 instrument
Foodproof® is a trademark of Biotecon Diagnostics
LightCycler® is a trademark of Roche Diagnostics
TaqMan® is a trademark of Roche Diagnostics
NOTE: This item is from our 'historic' database and may contain information which is not up to date.
Source : Merck Millipore View Company Information
Posted on October 27, 2008