Condagene^® ViableCell is a reagent intended to neutralize DNA from non-viable cells and/or free DNA that could lead to false positives in PCR-based detection techniques. This reagent cannot cross entire cell membranes associated with cell viability. The results obtained from PCR in samples treated with Condagene^® ViableCell are indicators of the presence of viable cells.

Viability PCR (v-PCR) performed using Condagene^® ViableCell in a wide range of food and water samples is a reliable and culture-free method that offers sensitive and rapid detection of viable microorganisms. It is a photoreactive, membrane-impermeant, DNA-binding dye that offers superior dead cell selectivity over traditional culture-based methods. The sample is left in contact with the reagent for 30 minutes in the dark; after that, the sample is photoactivated with blue light for 15 minutes. DNA that has encountered the reagent is covalently bound to it, ceasing to be a suitable template for PCR, so only DNA from viable cells will be detected on subsequent PCR.

• Sensitive, rapid method vs time-consuming culturing, microscopy or colony counting.
• Reliable: extensive bibliography tested and published for a wide range of microorganisms.
• Compatible with Next-Gen Sequencing applications.
• Suitable for complex sample types
• Easy to use: analyze samples easily and with minimal manipulation.
• Open system: Condagene is optimized for use with Condagene Extraction and qPCR Kits, and works with different protocols.