CondaChrome® TBX Agar
Principle: Escherichia coli colonies appear blue-green due to glucuronidase enzyme activity
Approvals: Designed following ISO 16649
Suitability: Selective detection and enumeration of E. coli in food for human and animal consumption
Capital equipment required: No
CondaChrome® TBX Agar is based on Tryptone Bile Salts Agar medium, used to detect and enumerate E. coli in foods, with the addition of a chromogenic agent, x-ß-D-Glucuronide, to detect the presence of the enzyme glucuronidase, which is highly specific for E. coli.
The released chromophore in TBX Agar is colored and target colonies are easily identified. E. coli absorbs the chromogenic agent x-ß-D-glucuronide, and the intracellular glucuronidase enzyme activity breaks the bond between the chromophore and the glucuronide. The released chromophore is colored and builds up within the cells, causing the E. coli colonies to be blue-green colored.
Casein peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Bile Salts are inhibitors to other Gram-positive organisms and suppress coliform bacteria. Bacteriological agar is the solidifying agent.
- High specificity: identification based on specific enzymatic activity of each microorganism
- Quick results: read your results after 24 hours, reduce drastically the time to results
- Easy interpretation: identify different bacteria by its characteristic color
- Time and space saving: detect several bacterial species in the same plate
- Minimum investment: no additional equipment nor special training required
- Formula and QC according to ISO standards