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14th August 2012  Content supplied by: MWE

Sigma-Virocult® - The Challenge of H3N2v

Sigma-Virocult® The current concern in North America regarding increased numbers of cases of H3N2v variant seasonal influenza A virus (with the influenza A (H1N1)pdm09 M gene) is a reminder of the need for constant vigilance against this cunning pathogen with its ability to alter without warning, and with renewed potential for serious outbreaks.

A new report from the United States Centers for Disease Control and Prevention 1 shows that in an evaluation of seven widely used FDA-cleared rapid influenza diagnostic tests against a panel of seven H3N2v strains , only four of the tests detected all of the strains. One of the rapid tests detected only one of the seven strains.

In the present situation with an emerging potentially virulent strain, it is essential to accurately identify all occurrences. The CDC report and others 2-5 have shown that the sensitivity of rapid influenza diagnostic tests can be low compared with virus isolation or RT-PCR. So where possible, all specimens where H3N2v is suspected should be confirmed by culture or RT-PCR.

For all of these techniques, a reliable transport device is essential, one which preserves the integrity and infectivity of virus and genome. In these circumstances the most trusted device is Sigma-Virocult®. In a study 6 presented at the 2010 Clinical Virology Symposium, Sigma-Virocult® was shown to maintain the viability of seasonal influenza A H3N2 for at least 14 days. The integrity of the virus genome, as demonstrated by RT-PCR was also maintained for at least 14 days.


Sigma-Virocult® is readily available around the world. Please contact us for details of your nearest distributor.


1. Centers for Disease Control and Prevention, Evaluation of Rapid Influenza Diagnostic Tests for Influenza A (H3N2)v Virus and Updated Case Count - United States, 2012, Morbidity and Mortality Weekly Report Early Release / Vol. 61 August 10, 2012

2. Hurt AC, Alexander R, Hibbert J, Deed N, Barr IG. Performance of six influenza rapid tests in detecting human influenza in clinical specimens. J Clin Virol 2007;39:132-5.

3. Faix DJ, Sherman SS, Waterman SH. Rapid-test sensitivity for novel swine-origin influenza A (H1N1) virus in humans. N Engl J Med 2009;361:728-9.

4. Ginocchio CC, Zhang F, Manji R, et al. Evaluation of multiple test methods for the detection of the novel 2009 influenza A (H1N1) during the New York City outbreak. J Clin Virol 2009;45:191-5.

5. Uyeki TM, Prasad R, Vukotich C, et al. Low sensitivity of rapid diagnostic test for influenza. Clin Infect Dis 2009;48:e89-92.

6. Valette, M., Bouscambert-Duchamp, M., Fanget, R., Lambert, S., & Lina, B., Comparison Of Virocult® Swab, S-Swab® And S-Virocult® For Influenza A Viability For Cell Culture And Molecular Detection, Poster S84, Clinical Virology Symposium 2010, Daytona Beach. Click here for further information.

7. And also at

9. Variant (Swine Origin) Influenza Viruses in Humans

Order Details

Product CodeProduct name Configuration
MW951SSigma-Virocult®1 standard Sigma-swab® with breakpoint
MW951SENTSigma-Virocult®1 mini-tip Sigma-swab® with breakpoint
MW951S2*Sigma-Virocult®2 standard Sigma-swabs® with breakpoint

*Other variants are available. Please check website.

In the United States Sigma-Virocult is available through Fisher Scientific:

Further details from

Sigma-Virocult® Brochure


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Date Published: 14th August 2012

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