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5th November 2019  Content supplied by: Bio-Rad Laboratories

Bio-Rad’s Listeria AFNOR Update

RAPID'L.mono Chromogenic Medium: New Confirmation Options

While current classical methods for Listeria detection are laborious and time-consuming, RAPID'L.mono medium allows the direct detection and enumeration of Listeria monocytogenes and Listeria spp. in food and environmental samples, with a fast, simple and cost-effective protocol.

On October 4, 2019, a new extension has been approved by AFNOR certification according to ISO 16140-2:2016 requirements, allowing two new confirmation options dedicated for Listeria genus by spotting on Palcam or AL.

These new options are easy to perform and allow the confirmation of up to 15 positive samples onto one Palcam or AL plate, making this a very flexible and cost-effective solution.

At the same time, the extension of NF VALIDATION certification has been granted for AL Enumeration and Detection alternative methods for Listeria monocytogenes with the Rhamnose test.

This new confirmation option, easy to handle and easy to read, allows the confirmation of the positive samples in only 6 hr. For more flexibility, the reading step can also be performed after 72 hr.  This is an easy option for the differentiation of Listeria monocytogenes and Listeria ivanovii. Confirmation can be performed either with the standard method, spot on Palcam or with real-time PCR.

 iQ-Check Listeria spp. and iQ-Check Listeria monocytogenes Renewal and Extension

NF VALIDATION has been renewed for iQ-Check Listeria spp and iQ-Check Listeria monocytogenes following the ISO16140-2 compared to the reference method ISO 11290-1 for the detection of Listeria spp. and Listeria monocytogenes in all human food products and industrial production environmental samples.  This validation extension for the production environmental samples category includes:

  • The reduction of the enrichment time to 18 - 26 hr (25% time reduction)
  • The use of the iQ-Check Free DNA Removal Solution (removal of signal from free DNA in the sample)
  • The use of a new Application Protocol File (APF) that significantly reduces PCR run time (38% time reduction)

Overall, these validation extensions provide increased flexibility for users as well as improved performance.

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Date Published: 5th November 2019

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