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24th February 2014  Content supplied by: ATCC

ATCC mycoplasma DNA Certified Reference Materials

mycoplasma contamination affects roughly 15-35% of continuous cell cultures, resulting in a number of deleterious effects including the induction of chromosomal abnormalities, the disruption of DNA and RNA synthesis, decreased transfection rates, and the inhibition of both cell metabolism and growth rate. To protect the quality of your cell cultures, routine testing for mycoplasma contamination is recommended.

To facilitate the rapid and reliable detection of mycoplasma in cell culture, ATCC offers a number of products and services, including the recent release of mycoplasma DNA certified reference materials. These tools are ideal for use in:

  • Inclusivity/exclusivity testing
  • Establishing limits of detection
  • Validating or comparing test methods

These nucleic acid preparations were isolated from common cell culture contaminants, and were quantified for genome copy number using Droplet Digital™ PCR. Moreover, as certified reference materials, each product has been prepared under an ISO Guide 34 accredited process to confirm identity, well-defined characteristics, and an established chain of custody.

Learn more about how ATCC can help you protect your cell cultures from mycoplasma contamination!

ATCC® No.SpeciesDesignation
qCRM-15531DMycoplasma pneumoniaeFH strain of Eaton Agent [NCTC 10119]
qCRM-17981DMycoplasma hyorhinisBTS-7 [ATCC 23234, PG 42, NCTC 10130]
qCRM-19989DMycoplasma fermentansPG18 [G, NCTC 10117]
qCRM-23064DMycoplamsa salivarium[H110, NCTC 10113, PG 20]
qCRM-23206DAcholeplasma laidlawiiPG8 [NCTC 10116, PG8, A]
qCRM-23714DMycoplasma oraleCH 19299 [NCTC 10112]
qCRM-23838DMycoplasma argininiG230 [NCTC 10129]
qCRM-25204DMycoplasma synoviaeWVU 1853 [NCTC 10124]
qCRM-27545DMycoplasma hominis[LBD-4]


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Date Published: 24th February 2014

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