Anaerobic Storage of Water Testing Plates for the Enumeration of Clostridium spp. to Meet New UK Guidelines
In the new UK methods for the isolation and enumeration of sulphite-reducing clostridia by membrane filtration (The Microbiology of Drinking Water (2004) - Part 6 - Methods for the isolation and enumeration of sulphite-reducing clostridia and Clostridium perfringens by membrane filtration. Methods for the examination of waters and associated materials. Environment Agency) it is recommended that prepared Tryptose Sulphite Cycloserine (TSC) agar plates (such as Thermo Scientific, CM0587 and SR0088E or PO0520A) are stored in a refrigerator (2-8°C) under anaerobic conditions for up to one week prior to use.
The recommendations state that the performance of TSC medium deteriorates during storage due to exposure to oxygen. This can be reversed by pre-reducing the medium prior to use, by storing the plates under anaerobic conditions, for up to a week. Therefore, anaerobic and refrigerated storage is important to maintain the quality and reliability of results.
Source : Thermo Scientific. View Company Information
Posted on July 29, 2004