Millipore Sigma Webinar

Salmonella Detection in Food

The revised ISO 6579 method for detection of Salmonella

The horizontal method for the detection of Salmonella spp. in food and animal feeding stuffs ISO 6579 1993 has been dramatically revised as Figure 1 shows.

One of the changes includes the replacement of Selenite Cystine Broth by a new Muller Kaufmann Tetrathionate Broth formulation with Novobiocin.

This new Muller Kaufmann Tetrathionate Broth is unique as the selective components are included in the base media and it's granulated so easy to prepare!

  • no dust
  • no more sticking
  • quick dissolving
  • no preparation of the supplement
  • no additions to the prepared basal medium
New Muller Kaufmann Tetrathionate Broth
Pre enrichment Buffered peptone water MERC 1.07228.0500/5000
Secondary selective enrichment Rappaport Vassiliadis peptone broth MERC 1.07700.0500
  Muller-Kaufmann tetrathionate broth
with Novobiocin (MKTTn)
MERC 1.05878.0500
Plating agar Obligatory XLD agar MERC 1.05287.0500
  Free choice Rambach agar MERC 1.07500.0003
  XLT- 4 agar MERC 1.13919.0500

Figure 1 Horizontal method for the detection of Salmonella ISO 6579 2002
The major changes in ISO 6579 from 1993 to 2002 version in red
ISO Standard 6579 2002

NOTE: This item is from our 'historic' database and may contain information which is not up to date.

Source : VWR International Ltd. View Company Information

Posted on September 15, 2003

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