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Merck's Sputofluol® Pretreatment Solution for TB Testing
Merck's Sputofluol® for Clean and Fast results
Sputofluol® is a pretreatment solution for use with sputum, FNAB, lavages, imprints, body fluids, exudates, puss, liquid, or solid cultures.
Sputum can be pretreated with Sputofluol® in order to release any mycobacteria from surrounding mucus making the final slide easier to read. The active ingredient in Sputofluol® is hypochlorite, which oxidatively dissolves the organic material whilst having little effect on the mycobacteria.
In a centrifuge tube mix 1 part of the sample (at least 2 ml) with 3 parts of a 15% Sputofluol® solution prepared with distilled water, and leave to react for 10 min shaking vigorously from time to time. Centrifuge at 3000-4800 U/ min for 20 min, decant the supernatant, smear out the sediment, allow to dry and follow the instructions for the staining procedure.
Sediments from punction and lavage material, are used in the following way - after carrying out the appropriate enrichment procedures smear the sample onto a microscope slide, allow to dry and follow the instructions for the staining procedure.
Accurate heat fixation of pretreated material is essential.
Tb-color staining set (cold stain)
sufficient for 250 preparations specimens
Tb-color modified staining set (hot stain)
sufficient for 250 prepared specimens
Tb-fluor phenol-free staining set (fluorescence stain)
sufficient for 50-60 prepared specimens
100 ml, 500 ml
Immersion oil acc. to DIN ISO 8036-mod.
Oil of cedar wood
100 ml, 500 ml
For further information about the wide range of products available from Merck Millipore for TB detection, please contact Merck Millipore (see details above) or EMD Chemicals in the USA or click the 'More Information' button below.
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