New! Confirmation of Listeria monocytogenes in 6 h With a Single Broth
The principle behind the RAPID'L.mono medium relies on the specific detection of L. monocytogenes phospholipase C (PIPLC) and on the inability of this species to metabolize xylose.
After 24 hours of incubation, Listeria monocytogenes forms characteristic blue (pale blue, grey-blue to dark blue) colonies without a yellow halo. Colonies formed by other species of Listeria are white, with or without a yellow halo.
The characteristic of the species Listeria ivanovii, infrequently found in food matrices, should be noted: they present blue-green colonies with a yellow halo (indicating xylose positive ). This halo can appear after 24 to 48 hours of incubation.
The selective solution in the medium inhibits interfering flora (Gram-positive and Gram-negative bacteria, yeasts and molds).
RAPID'L.mono: 24h enrichment + 24h detection + 6h confirmation
Now, for faster confirmation and differentiation of Listeria monocytogenes and Listeria ivanovii, the Rhamnose test was certified by AFNOR certification for a confirmation of positive results from RAPID'L.mono strains in 6 hours.
Source : Bio-Rad Laboratories, S.A. View Company Information
Posted on March 16, 2009