16th April 2015 Content supplied by: Wako Chemicals USA, Inc.
Limulus Color KY Series Can Detect Endotoxin to 0.0002EU/ml
The Limulus Color KY Series includes both a multi-test kit containing 60 tests and a single-test kit containing 25 tests, each designed for time-based colorimetric analysis, using a synthetic substrate which produces a yellow color and can specifically detect endotoxin with high sensitivity.
The reagent is based on the color development mechanism, which is activated by the presence of endotoxin. First, a series of activations of serine protease precursors in the reagent occur, consequently triggering the final reaction, in which a clotting enzyme hydrolyzes the chromogenic substrate to release a yellow chromogen (pNA). Since (1→3)-β-D-glucan, when not present in high concentrations, has also been found to activate the LAL, Wako has incorporated a large amount of (1→3)-β-D-glucan into the Limulus Color KY reagent so that the activation by (1→3)-β-D-glucan is completely inhibited and therefore allows the endotoxins to be specifically assayed.
- Avoids false positive results
- Available in multi-tests vials or single-test vials
- Quantitative KCA reagent (kinetic-chromogenic assay) reagent
- KCA assays can be performed in tube reader or microplate reader
- KCA quantitative range detection limit of 0.0002 Eu/mL (single-type) and 0.0005 Eu/mL (multi-type).
- Available with matched control standard endotoxin (CSE)
Date Published: 16th April 2015
Source article link: http://www.wakopyrostar.com
PYROSTAR™ ES-F – Endotoxin Specific LAL
Limulus PS Overcomes Product Inhibition