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Real-Time PCR and Ch...

12th May 2020  Product update: Bio-Rad Laboratories

Bio-Rad’s Complete Solution for Legionella Testing

Bio-Rad provides a complete range of products for the detection and the quantification of Legionella. Products comply with regulatory standards and allow for risk management based on accurate and rapid results. These Legionella testing products are suitable for water supply systems, water intended for human consumption, hot water for sanitation purposes, water for industrial use, natural mineral water, water for recreational activities, biofilms, and aerosol samples. 

Real-time PCR  range includes iQ-Check Legionella spp. and L. pneumophilia kits, associated with the high performance Aquadien DNA Extraction kit

  • Compatible with all types and volume of water (water systems, drinking water as well as cooling tower water)
  • Delivering results in 4 hours from sample receipt with no confirmation needed
  • Can detect and quantify Legionella spp. and L. pneumophilia in the same assay
  • 96 well format allows for an adaptable number of assays per run
  • Fully automated results with internal positive and negative control
  • Free DNA Removal Solution option to improve the reliability of results
  • AFNOR VALIDATION certified according to NF T90-471 and ISO TS 12869
  • Reliable for colony confirmation according to NF T90-431 and NF EN ISO 11131

Key Benefits:

Fast - 4 hr from sample to results, No confirmation needed

Reliable - Detects all Legionella species including viable but nonculturable (VBNC) and intra-amoebal, includes positive/negative DNA controls and internal control, avoids contamination risk by not requiring post-PCR processing

Convenient - All reagents provided with the kits and ready-to-use, automated result interpretation

High Performance - NF VALIDATION certified according to the T90-471 and ISO/TS 12869 standards, Gene-specific primers and probes for maximum specificity, quantification standards connected international standard reference material.

Adding the non-toxic and easy to use Free DNA Removal Solution (FDRS) step to the Aquadien DNA Extraction/Purification Kit removes free DNA that is present in water samples, leading to equivalency between PCR and culture results when VBNC cells are not present.

Watch Bio-Rad's Recorded Webinar on Legionella Testing Solutions   

Traditional Culture Media and Confirmation - In addition to the iQ-Check real-time PCR range, Bio-Rad also has a range of Legionella culture media and confirmation kits including:

  • BCYE agar +/- L-Cysteine
  • Columbia Agar + Horse Blood Agar
  • GVPC Agar
  • Legionella pneumophilia and Legionella spp. Latex Kit
  • Legionella DNA Free Water
  • MonoFluo Kit for L. pneumophilia

For more information on Bio-Rad's water testing solutions, visit

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Date Published: 12th May 2020

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Note: This content has been edited by a rapidmicrobiology staff writer for style and content.

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