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Enhanced Identification of Lactose-Fermenting Organisms

Thermo Scientific Limited has launched a new pack size of 5% tri-phenytetrazolium chloride (TTC) Solution (SR0211C) supplement for addition to Tergitol-7-Agar (CM0793) and KF Streptococcus Agar (CM0701). This supplement is rapidly reduced to insoluble red formazan providing an early indication of bacterial growth.

Tergitol-7 Agar is a selective medium for the detection and enumeration of coliforms in food and water samples. It inhibits Gram- positive organisms and minimises swarming of Proteus spp., allowing superior recovery of coliforms.

The addition of TTC to this medium allows earlier recognition and identification of E. coli and Enterobacter aerogenes . Unlike other coliforms, these species do not produce red formazan due to their rapid lactose fermentation.

KF Streptococcus Agar is recommended for the detection and enumeration of enterococci in milk, water and other materials, thus serving as an indicator of faecal contamination. The addition of TTC to this medium enhances enumeration by causing enterococci colonies to be red or pink in appearance.

For further information about the use of Thermo Scientific 5% TTC solution, Tergitol-7 Agar
and KF Streptococcus Agar:

Telephone: +44 (0) 1256 841144
Fax: +44 (0) 1256 329728
Email: leslie.bissell@thermofisher.com
or visit the Thermo Scientific website at: http://www.oxoid.com/