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Merck's Complete Medium for ISO 16649-1 Resuscitation of Sublethally Injured E. coli

When testing foodstuffs or raw materials for Escherichia coli, it is mandatory to include a resuscitation step using MMGA (Mineral Modified Glutamate Agar) to increase food safety and protect public health. Only MMGA from Merck contains everything you need in a ready to use format.
  • Reliable
    All E. coli can be detected, both injured and non-injured.
  • Easy
    MMGA from Merck is a complete medium; nothing needs to be added.
  • Fast
    Only 4 hours
    resuscitation.
  • Safe
    Granulated medium for a safer and cleaner working environment.

Legally required: E. coli resuscitation
Since January 1, 2006, European regulation on microbiological criteria for foodstuffs references the ISO standard 16649 as mandatory for the detection of E. coli in certain foods. Part 1 of this standard describes a membrane method that includes a resuscitation step for E. coli. This step is necessary when the food has undergone processes such as heating, drying, chemical preservation, freeze-drying, or acidification, since these treatments can damage and injure any bacteria present. Using the standard detection medium for E. coli, i.e. TBX agar, with its high amount of selective substances and high incubation temperature of 44°C without resuscitation, it is possible to get false negative results as injured E. coli are unable grow reliably. This is also true for raw materials subjected to processing.

Reliable and safe: ISO-compliant MMGA from Merck
To minimize risk, the ISO standard recommends the use of MMGA for this recovery step. For your convenience, the MMGA medium from Merck offers a unique combination of glutamate, selected minerals and agar, ensuring a very rapid and effective resuscitation of injured E. coli. With our product, most of the bacteria are sufficiently repaired after incubation of only 4 hours at 37°C, so that reliable growth on ChromoCult® TBX agar at 44°C is guaranteed.

As usual, Merck's MMGA medium is provided in granulated form in order to increase safety in your laboratory. This drastically diminishes dust development and minimizes the risk to lab personnel of inhaling potentially hazardous substances.

Complete medium for resuscitation
Only MMGA from Merck contains everything you need for resuscitation of sublethally injured E. coli according to ISO 16649-1. If food business operators use, for example, DEV glutamate broth to prepare the MMGA, the agar still needs to be added. Other media even require the addition of a glutamate solution. Containing glutamate, minerals, and agar, MMGA from Merck is the complete and ready-to-use solution.

Effect of MMGA on recovery of heat-stressed cells of E. coli ATCC 25922
(2 min at 55°C in a water bath)

E.coli without resuscitation
TBX agar (44°C)
without resuscitation on MMGA
E.coli with resuscitation
TBX agar (44°C)
with resuscitation on MMGA


Available Products Merck Cat. No.
MMGA 1.09045.0500
ChromoCult® TBX 1.16122.0500


For further information about MMGA please contact Merck Millipore (see details above) or EMD Chemicals in the USA or click the link below.
NOTE: This item is from our 'historic' database and may contain information which is not up to date.

Source: Merck Millipore View latest company information

Posted: May 9, 2008
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