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31st January 2017  Content supplied by: 3M Food Safety

Which Molecular Test Gives Fastest Time to Result for Salmonella?


3M Food Safety have announced the results of a scientific study conducted by a third party ISO/IEC 17025 accredited laboratory. Cost and time associated with performing each platform’s protocol was modeled (evaluated) by comparing workflows. The research found that the 3M™ Molecular Detection Assay 2 – Salmonella test saved significant hours and cost versus three other common pathogen detection platforms included in the study.

For each of the four pathogen detection platforms, a total of 96 samples of ground poultry were evaluated in duplicate, resulting in 192 detection method tests per platform, for a total of 768 results. Time was measured on the aggregate and according to the following parts of the detection protocol: sample preparation; instrument and software set up; sample lysis/capture; and assay setup. The study focuses largely on speed because turnaround time significantly affects food production and testing, particularly when a producer’s goods are perishable or in high demand. While the introduction of any rapid microbiology method should have a positive impact, there are still distinctions between each method.

Total time to result for the detection of Salmonella by four different platforms in 96 samples.

Method Steps

Time to Result (minutes)a

3M™ MDA2

Technology 1*

Technology 2*

Technology 3*

Detection method set up

31.7

37.3

63.9

84.8

Lysis/capture  incubation +Instrument run time

80

240

124

207

TOTAL

111.7

277.3

187.9

291.8

 

aPreparation of sample including weighing 25 g of sample and adding 225 mL of culture media was similar for all four methods, at approx. 116 min

*Technology: Commercial method available in the marketplace

Based on the findings of this study, using the 3M Molecular Detection Assay 2 – Salmonella method to perform 10,000 Salmonella tests would save a laboratory between 132 and 312 labor hours annually as compared to the other detection platforms, up to 2.6 times faster, which would potentially result in an annual cost savings of up to $6,240.

To download the complete study and view the accompanying infographic, click here.

For more information about the 3M Molecular Detection System, visit 3M.com/foodsafety/MDA2 or follow @3MFoodSafety on Twitter.


    

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Date Published: 31st January 2017

Source article link: http://www.3m.com/3M/en_US/food-safety-us/resources/testing-efficiency/


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