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31st August 2021 Content supplied by: Pall Corporation
PCR Needs Easy and Highly-Efficient Instrument for DNA Purification
PCR (Polymerase Chain Reaction) has never been this easy.
The Pall GeneDisc system is a complete, rapid, reliable, and easy-to-use solution for microbial testing in three simple steps from sample to result. After samples are extracted with the GeneDisc Ultra-Lyser, the DNA may be purified by utilizing the GeneDisc Ultra-Purifier. Extracted and purified DNA is then loaded into a GeneDisc Plate designed for specific detection and/or quantification of microorganisms of interest. The GeneDisc Plate is then placed into the GeneDisc Cycler for analysis. The analysis step by real-time Polymerase Chain Reaction (RT-PCR) runs in as little as an hour and the final results are displayed with no further operator intervention.
For specific applications, such as quantification of Legionella in complex waters, or to detect and quantify the presence of pathogens such as E. coli and Enterococcus spp. within three hours, DNA purification is a key step of sample preparation. DNA purification enables:
- To reduce PCR inhibitions due to organic compounds from samples
- To achieve highly accurate results.
The GeneDisc Ultra-Purifier ensures high-quality DNA purification based on the technology of DNA molecules adsorption to silica and solvent washings. This protocol, applied to GeneDisc methods for Legionella, was validated by NF VALIDATION against NF T90-471 and ISO /TS 12869 standards.
For easy, reliable microbial test results without the wait click here or contact the supplier using the green "Request Information" button below.
Date Published: 31st August 2021
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