Workshop on Legionella Quantification in 4 Hours - Potsdam, Germany
: 2 Mar 2018
: Potsdam, Germany
This workshop will focus on understanding the basics of Legionella testing and is intended to provide the audience with information about the requirements, regulations and methods for Legionella testing. Real-time PCR and cultural methods (ISO 11731:2017) are compared. A practical training in our laboratory will help to understand how real-time PCR can be used for the specific individual quantification of Legionella spp., Legionella pneumophila and Legionella pneumophila serogroup 1. The hands-on training will guide through all steps of the analysis, starting with filtration, followed by live/dead differentiation, DNA extraction and real-time PCR setup. Finally, result interpretation and reporting complete the workshop.
The workshop is addressed to those involved in the management and control of Legionella, who are just implementing real-time PCR in the laboratory or already use real-time PCR and are looking for the best method.
Topics of the Workshop:
- Requirements, regulations and methods for Legionella testing
- Comparison of real-time PCR and cultural methods (ISO 11731:2017)
- Practical training in our laboratory: filtration, live / dead differentiation and real-time PCR
- Result interpretation and reporting