You will learn about the general principles of real-time PCR, including experiment setup and data analysis. Afterwards you receive practical hands-on training for DNA-extraction and real-time PCR setup using the BIOTECON Diagnostics foodproof ® kits. All necessary materials and equipment will be provided on site.

Furthermore, you get the opportunity to familiarize yourself with different real-time PCR cyclers, such as the PikoRealTM24 (for low throughput), the AriaMx, the Lightcycler ®96 and the Lightcycler ®480 II and receive training on them. You will learn about technical specifications of the cyclers, handling and the corresponding software systems. A detailed data analysis of the acquired real-time PCR data is included.

Topics of the Workshop:

• Introduction and theory of real-time PCR
• Applications for quantitative and qualitative analysis
• Practical hands-on training for DNA extraction and quantitative PCR setup using different real-time PCR cyclers
• Data analysis and result interpretation
• Troubleshooting discussion

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