Salmonella Detection in Poultry and Salmonella Vaccines
: 28 Nov 2018
: Potsdam, Germany
Sector: Food & Beverage
Salmonella are rod-shaped, Gram-negative bacteria. The genus Salmonella is assigned to the family Enterobacteriaceae. They cause disease in humans and animals, including typhoid fever and diarrhea. Infection commonly occurs through ingestion of contaminated food, typically eggs and poultry. Enhanced Salmonella programs in poultry were implemented in all EU Member States and targets were set for reducing the bacteria in poultry flocks (laying hens, broilers and turkeys). The use of vaccines against Salmonella is an example of such potential specific control methods. Both live and inactivated Salmonella vaccines are available. The vaccines are targeted for the most commonly reported serovars of human infections in Europe (S. Enteritidis andS. Typhimurium).
This workshop will focus on understanding the basics of Salmonella testing. Real-time PCR and cultural methods are compared. A practical training in our laboratory will help to understand how real-time PCR can be used for the detection of Salmonella spp. We will also discuss qualitative detection of the live vaccine strain Salmonella Enteritidis 441/014 and the differentiation of the serotypes Enteritidis and Typhimurium.
Topics of the Workshop:
- Introduction and theory of Salmonella spp. detection by real-time PCR
- Hands-on training of real-time PCR in the laboratory
- Data analysis and result interpretation
- In-depth discussion about detection of live vaccine strains and differentiation of the serotypes Enteritidis (SE) and Typhimurium (ST)