New oxoid medium enhances the identification of pseudomonas aeruginosa
| Pseudomonas aeruginosa on Thermo Scientific Pseudomonas Cetrimide Agar (CM579) |
Thermo Scientific Limited has launched a new selective culture medium for the isolation and identification of Pseudomonas aeruginosa . New Thermo Scientific Pseudomonas Cetrimide Agar (CM579) exploits and enhances the ability of Pseudomonas aeruginosa to produce coloured and fluorescent water-soluble iron chelators, allowing the presence of the organism to be easily visualised on an agar plate.
The addition of Cetrimide inhibits the growth of other micro-organisms whilst magnesium chloride and potassium sulphate in the medium enhance the production of pyoverdin and pyocyanin in Pseudomonas aeruginosa. If Pseudomonas aeruginosa is present, this results in yellow-green or yellow-brown colonies that fluoresce under UV light.
Pseudomonas Cetrmide Agar is recommended by both the United States Pharmacopoeia (USP) XXVI  and the European Pharmacopoeia (EP) IV  for use in Microbial Limit Tests. In addition, this medium is recommended in A.O.A.C. guidelines  for the isolation of Pseudomonas aeruginosa from cosmetics and in the A. O. A. C method for testing disinfectants on hard surfaces [3,4].
To find out more about Thermo Scientific Pseudomonas Cetrimide Agar and other products in the Thermo Scientific range for the growth and identification of Pseudomonas aeruginosa:
Telephone: +44 1256 841144
Fax: +44 1256 463388
Email: firstname.lastname@example.org or visit the Thermo Scientific website at http://www.oxoid.com/
 United States Pharmacopoeia 2002. Microbiological Limit Tests, United States Pharmacopoeia, 26th Edition. United States Pharmacopial Convention, Rockville, M.D.
 European Pharmacopoeia 2002. Microbial Examination of Non-Sterile Products (Tests for Specified Microorganisms). European Pharmacopoeia 4th Edition.
 Association of Official Analytical Chemists 1995. Bacteriological Analytical Manual, 8th Edition. A.O.A.C. International, Gaithersburg, M.D.
 Official Methods of Analysis of A.O.A.C. International, 17th Edition, Revision 1, 2002.
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