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Salmonella Detection in Food

The revised ISO 6579 method for detection of Salmonella

The horizontal method for the detection of Salmonella spp. in food and animal feeding stuffs ISO 6579 1993 has been dramatically revised as Figure 1 shows.

One of the changes includes the replacement of Selenite Cystine Broth by a new Muller Kaufmann Tetrathionate Broth formulation with Novobiocin.

This new Muller Kaufmann Tetrathionate Broth is unique as the selective components are included in the base media and it's granulated so easy to prepare!

no dust
no more sticking
quick dissolving
no preparation of the supplement
no additions to the prepared basal medium

Pre enrichment	Buffered peptone water	MERC 1.07228.0500/5000
Secondary selective enrichment	Rappaport Vassiliadis peptone broth	MERC 1.07700.0500
	Muller-Kaufmann tetrathionate broth with Novobiocin (MKTTn)	MERC 1.05878.0500
Plating agar	Obligatory XLD agar	MERC 1.05287.0500
	Free choice Rambach agar	MERC 1.07500.0003
	XLT- 4 agar	MERC 1.13919.0500

Figure 1 Horizontal method for the detection of Salmonella ISO 6579 2002
The major changes in ISO 6579 from 1993 to 2002 version in red ISO Standard 6579 2002