Effective Solutions for Isolation and Concentration of E.coli O157
Invitrogen has effective solutions for the concentration and isolation of E. coli (Product) and other bacteria. The recent outbreak of E coli (EHC/VTEC) infections in Europe and the US highlights the need for an effective means of isolating E. coli (EHC/VTEC) from food and environmental samples.
Please see the Invitrogen standard method for E. coli (EHC/VTEC) Method, and the Invitrogen E. coli (EHC/VTEC) Publications listing. The Dynal products are already quoted in the USFDA Bacteria Analytical Methods Online , where it is stated that "Additional selective enrichment by use of immunomagnetic separation (IMS) has been found to be useful in the analysis of some foods, particularly those with high levels of competing microbial flora (29). Anti-O157 immunomagnetic beads (Dynabeads)."
Many people will pair IMS to the front end of PCR to eliminate indeterminate results and decrease overall pre-enrichment time. The additional bonus with IMS is that you can reserve an aliquot for culture confirmation for any PCR positive results.
Source: Invitrogen Ltd [USA]
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Posted: September 27, 2006
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