Comparison Table of Genotypic Microbial Identification or Typing Systems for Routine Use

Manufacturer
Applied Biosystems
Sequenom
bioMérieux
DuPont Qualicon
Intended Use Identification Identification Typing Typing
Product MicroSEQ® Microbial Identification System MassARRAY® System with iSEQ ™ software - Comparative Sequence Analyser Diversilab™ System
RiboPrinter®
Microbial Characterization System
Principle
  • DNA extraction from a single colony


  • PCR and cycle sequencing


  • Automated 16S rDNA and D2 LSU sequence analysis and identification
    		•  PCR of target region, transcription with T7 and SP6 polymerases base-specific RNA cleavage analysed by MALDI-TOF mass spectrometry. DNA extraction followed by repetitive sequence-based PCR (rep-PCR), fragments separated by electrophoresis in a microfluidic DNA LabChip, fragments bind with intercalating dye and detected by laser. southern hydridization
    restriction enzymes cleave extracted DNA, DNA extraction followed by PCR of 16s rRNA gene fragments separated by gel electrophoresis then hybridized with chemiluminescent DNA probes camera records light emissions need multiple restriction endonucleases for charting similarity of isolates.
    Results DNA sequence comparison to identification databases, with phylogenetic analysis. compared to database for identification, detects single base pair deviations and identifies new sequences, produces distance matrices - sample clustering. Strain and sub-species discrimination. Similarity matrix and dendrogram available for determining strain relatedness. Databases available for characterization RiboPrint® pattern compared to database for characterization or compares identification at subspecies level.
    Fastest time to result 5 hours 1 hour 5 hours 8 hours
    Max no. tests per day 80 3072 48 32
    Library 1900 bacterial species
    1000 fungal species    		 open database enables comparative sequencing with other reference databases bacteria, viruses, fungi. Over 3000 bacterial fingerprints
    Over 500 fungal fingerprints    		 6900 patterns
    219 bacterial genera
    1440 species and serotypes.



    Table prepared Aug 2008.
    Whilst every effort is made to ensure the information here is up to date, it should be used for guide purposes only, we cannot accept any responsibility for incorrect data. Data has been provided by the manufacturers.