Single-Stage Salmonella Enrichment Extension AOAC-RI Validated
The AOAC Research Institute has approved a method extension for the DuPont™ BAX® System real-time PCR assay for Salmonella to include enrichment protocols using Actero™ Salmonella Enrichment Media from FoodChek™ Systems. Included in this method extension are protocols for two new sample types – milk chocolate and chocolate liquor – and shortened protocols for dry pet food and environmental sponges.
“Chocolate products have become a higher-priority food type for Salmonella testing, but are difficult to test with rapid molecular methods due to the inhibitory properties of chocolate,” said Morgan Wallace, DuPont Nutrition & Health senior microbiologist and validations leader for Diagnostics. “By combining the robust formulation of Actero™ media for enhanced Salmonella enrichment and the sensitivity of PCR processing and automated detection with the BAX® System real-time PCR assay, this new AOAC-approved method for milk chocolate and chocolate liquor provides customers with an accurate, reliable testing method that can easily fit with a standard laboratory work flow.”
In addition to providing Salmonella testing of these new sample types, this proprietary media helps to provide faster, simpler enrichment procedures for testing products with the BAX® System method. “Actero™ Salmonella Enrichment Media reduces the total enrichment time for sample types such as pet food and environmental swabs by about 7-9 hours compared to enrichment with standard enrichment media. This time savings, combined with the reduced media volume required, can help improve food safety testing efficiencies and the financial bottom line for food companies and commercial testing laboratories alike,” said William J. Hogan, FoodChek™ chairman, president and CEO.
Tags: Salmonella, PCR, AOAC PTM, Salmonella in Chocolate, Salmonella in Pet Food
Date Published: September 21, 2015 » company contact details
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