Seward Stomacher® Used for Large Scale Campylobacter Study
Seward's Stomacher® technology is being used for the preparation of samples for a UK-wide Campylobacter study.
A recent survey of chicken meat purchased from major UK retailers has demonstrated the ubiquitous presence of Campylobacter species awaiting the unwary or poorly skilled cook. The project was undertaken by the UK Food Standards Agency (FSA) using both public and private microbiology laboratories to produce the data. The samples processed were chicken skin and sponge swabs taken from chicken carcasses. The FSA is working with government and industry to try to reduce the incidence of Campylobacter in chicken meat by 2015, a target the report suggests they will struggle to achieve1.
The Stomacher® paddle blender was used to process both chicken skin and sponge swabs in order to maximise bacterial recovery at the pre-enrichment phase, ensured by the Stomacher’s patented paddle design. Furthermore, the protocol adopted stressed the need for preventing cross contamination between samples which the Stomacher methodology also safeguards through use of irradiated sterile Stomacher bags. The sponge swab technique followed by extraction in the Stomacher has also been shown to be a superior method to swab sticks, generating a more representative result.2.
If elimination of Campylobacter is proving a challenge, then close monitoring and epidemiological studies will be keeping microbiologist busy for the foreseeable future. “The traditional techniques of horizontal isolation of Campylobacter are reliable but slow. New real-time PCR techniques require shorter pre-enrichment following Stomaching which could reduce time to result to just 24 hours, to potentially enable processors to clear products before shipping,” said Stuart Ray, Technical Director, Seward Ltd. “The quality of the sample produced by the Stomacher is essential for the reliability of this new approach.” A study of this technique applied to Salmonella in chicken demonstrates its advantages.3.
2) Thom K.A. et al.. Comparison of swab and sponge methodologies for identification of Acinetobacter baumannii from the hospital environment. Journal of Clinical Microbiology 2012, 50(6):2140.
Tags: Campylobacter, Sample Preparation
Date Published: March 2, 2015
Source article link: Seward Limited » company contact details
Why Bio-Rad? Learn More Here!
iQ-Check® Free DNA Removal Solution for Legionella
MWE Sigma Transwabs® and Fecal Transwabs® Compatible with Copan WASP®
Filter Plugs Ensure Contamination-Free Pipetting
Dedicated Serial Dilution Bags - Good for Staff, the Environment and Results