Rapid Detection of Salmonella Serovars Enteritidis and Typhimurium

Salmonella Enteritidis & Typhimurium Detection LyoKit

BIOTECON Diagnostics now offers a fast, sensitive and convenient assay for the detection and identification of the Salmonella serovars Enteritidis (SE) and Typhimurium (ST): The foodproof® Salmonella Enteritidis & Typhimurium Detection LyoKit, a lyophilized, pre-aliquoted rapid test for the identification of SE and ST in one assay.

The foodproof® Salmonella Enteritidis & Typhimurium Detection LyoKit is the first multiplex real-time PCR rapid test on the market, which detects SE and ST simultaneously in one single test in less than 24 hours. In combination with the foodproof® StarPrep One Kit for sample preparation, BIOTECON Diagnostics offers a safe, easy-to-use and time-saving solution.

The rapid assay has been validated with more than 110 SE and ST strains, including the monophasic variant 1,4,[5],12:i:-, in combination with all relevant matrices, e.g. egg shells, chocolate and cheese. For exclusivity more than 550 non-Enteritidis, non-Typhimurium Salmonella strains were successfully tested.

With this kit BIOTECON Diagnostics introduces the foodproof® LyoKits Product Series, designed to raise the convenience, sensitivity and safety of the foodproof® kits. The pre-filled reaction mix is lyophilized, and the DNA sample preparation can be added directly to each reaction tube. With less pipetting steps, the risk of cross-contamination has been further reduced. The LyoKits are easy to store at 2 - 8 °C and can be shipped at room temperature. Every LyoKit includes an internal amplification control and the proven Uracil-N-Glycosylase system, which prevents carry-over contamination by prior PCR amplificates. The LyoKits are compatible with nearly all open real-time PCR cyclers based on Hydrolysis Probes.

The foodproof® LyoKits Product Series will be expanded by further parameters; Salmonella, STEC and Listeria monocytogenes are already in the process to be launched.

Get the accuracy, the ease-of-use and a faster time-to-result with reduced overall testing costs, even with challenging samples.

Email BIOTECON Diagnostics GmbH at bcd@bc-diagnostics.com

More information on Salmonella Serovars S. Enteritidis and S. Typhimurium
:
About 80% of all Salmonella infections in humans are due to Salmonella enterica subspecies enterica serovar Enteritidis (SE) and Salmonella enterica subspecies enterica serovar Typhimurium (ST), according to the European Food Safety Authority. Salmonellosis in humans is often associated with consumption of poultry, eggs and egg-derived products. Additionally, infections in chickens, turkeys and ducks cause economic loss worldwide with morbidity of up to 85% and a low to moderate mortality. Usually, infected birds are culled or rejected at slaughter.

Because SE and ST are the predominant serovars associated with human disease in most countries and because of the differences in the epidemiology as compared to other Salmonellae, these are often specifically cited in zoonosis control legislation. For example, the EU Regulation 1086/2011 claims, that fresh poultry meat has to be free of SE and ST, including the monophasic Salmonella Typhimurium strain 1,4,[5],12:i:-.

The U.S. Food and Drug Administration (FDA) has issued the Final Rule “Prevention of Salmonella Enteritidis in Shell Eggs During Production, Storage, and Transportation”, that requires “shell egg producers to implement measures to prevent SE from contaminating eggs” (74 FR 33030). SE can infect the reproductive tract of chickens and enter shell eggs before the shell is formed. An accurate and sensitive test system is required, because levels of SE in eggs from infected hens are often low. The proposed FDA BAM method to test environmental and egg samples is time-consumptive and takes up to seven days for a negative result.


     
Tags: Listeria, Identification, Poultry, Salmonella, Sample Preparation, Listeria monocytogenes, PCR, FDA, Salmonella Typhi, Salmonella Typhimurium

Date Published: October 1, 2013

Source article link: Biotecon Diagnostics » company contact details

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