Discrimination of Infectious Hepatitis A Virus and Rotavirus by RT-qPCR
Human enteric viruses are major agents of foodborne diseases. Because of the absence of a reliable cell culture method for most of the enteric viruses involved in outbreaks, real-time reverse transcriptase PCR is now widely used for the detection of RNA viruses in food samples. However this approach detects viral nucleic acids of both infectious and non infectious viruses, which limits the impact of conclusions with regard to public health concern.
This study from the ANSES (French Agency for Food, Environmental and Occupational Health & Safety), looks at developing a method to discriminate between infectious and non-infectious particles of hepatitis A virus (HAV) and two strains of rotavirus (RV) following thermal inactivation by using intercalating dyes combined with RT-qPCR.
Tags: PCR, Rotavirus, Hepatitis A
Date Published: October 7, 2013
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