RIDASCREENŽ Campylobacter achieves significantly better recovery rates than conventional culture on Campylobacter agar, the method required for culture-based detection after selective pre-enrichment. This was confirmed in a national interlaboratory ring trial in Germany where the study was designed to validate PCR methods for the detection of Campylobacter in foods. The participants however were allowed to use the RIDASCREENŽ Campylobacter ELISA as an unofficial alternative to the culture-based method of confirming the test results.
Seven out of the nine laboratories participating in the study used the ELISA as an additional confirmatory test. Using 8 positive and 8 negative ground meat samples each, a total of 112 samples were tested with the RIDASCREENŽ Campylobacter ELISA. All negative samples inoculated with E. coli were confirmed negative by culture as well as by ELISA. Forty-eight of the 56 positive ground meat samples inoculated with C. jejuni, were tested by both culture and ELISA and were therefore available for comparison. RIDASCREENŽ Campylobacter identified 31 of the Campylobacter-inoculated samples as positive whereas culture identified only 21 as positive. Likewise, the recovery rate was only 43 % for the traditional culture method compared to 65 % for the immunological RIDASCREENŽ Campylobacter ELISA method.