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BBL™ CHROMagar™ Staph aureus Medium Receives AOAC™-RI Approval

BBL™ CHROMagar™ Staph aureus
For the Rapid Detection of Staph. aureus From Food

BBL CHROMagar Staph aureus has been validated by the AOAC™- Research Institute (AOAC-RI) under the Performance Tested Methods[SM] Program for the analysis of shell eggs, smoked salmon and cooked roast beef when using AOAC and ISO Official Methods.[1,2]

S. aureus is a well documented pathogen. It is responsible for infections ranging from superficial to systemic.[3,4] Due to the prevalence of this organism and its clinical implications, detection is of utmost importance. Staphylococcal food poisoning, caused by S. aureus, is one of the most common types of foodborne illnesses worldwide. Its detection and enumeration help provide information about the potential health hazard of food, as well as being an indicator of poor hygiene.[5]

BBL CHROMagar Staph aureus is intended for the isolation, enumeration and identification of S. aureus based on the formation of mauve-colored colonies. The addition of chromogenic substrates in the medium facilitates the differentiation of S. aureus from other organisms. The distinct advantage BBL CHROMagar Staph aureus has over traditional media, such as Baird-Parker Agar, is:

  • Identification of Staph. aureus in 24 hours as opposed to 48 hours for Baird Parker
  • Sensitivity 100% and specificity 100%[6]

Only BBL CHROMagar Formulations Have AOAC-RI Approval!

BBL™ CHROMagar™ Family

Cat. No.

Unit

BBL™ CHROMagar™ Listeria
215085
20 plates
BBL™ CHROMagar™ O157
214984
20 plates
BBL™ CHROMagar™ Salmonella
214983
20 plates
BBL™ CHROMagar™ Staph aureus**
214982
20 plates
Associated Products
Cat. No.
Unit
Difco™ Maximum Recovery Diluent 218971 500 g


**BBL™ CHROMagar™ Staph aureus is approved by Government of Canada, Health Products and Food Branch, 3rd Supplement, Method MFHPB-21, November 2003.


Reference
1. AOAC Official Method 9755.55. Staphylococcus aureus in foods. Surface plating method for isolation and enumeration. 1976.
2. International Organization for Standards (ISO). Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of coagulase-positive Staphylococci (Staphylococcus aureus and other species) - Part 1: Technique using Baird Parker agar medium, 1st ed., ISO 6888-1:1999.
3. Jablonski, L.M. and G.A. Bohach. 1997. Staphylococcus aureus. In M. Doyle, L. Beuchat and T. Montville (eds.), Food microbiology fundamentals and frontiers. ASM, Washington, DC.
4. Kloos, W.E. and T.L. Bannerman. 1999. Staphylococcus and Micrococcus. In P.R. Murray, E.J. Baron, M.A. Pfaller, F.C. Tenover and R.H. Yolken (eds.), Manual of clinical microbiology. ASM, Washington DC.
5. Bennett, R.W. and G.A. Lancette. 1998. Staphylococcus aureus. In FDA, bacteriological analytical manual, 8th ed. AOAC International, Gaithersburg, MD
6. Data on file, BD Diagnostic Systems.

AOAC is a trademark and Performance Tested Methods Program is a service mark of AOAC International. CHROMagar is a trademark of Dr. A. Rambach. Difco is a trademark of Difco Laboratories, Inc., a subsidiary of Becton, Dickinson and Company. BD, BD Logo and BBL are trademarks of Becton, Dickinson and Company. ©2005 BD

Source: BD Diagnostic Systems
Posted: November 4, 2005

March 19, 2010
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